E-Selectin/CD62E 蛋白, Mouse (HEK293, His)

E-Selectin/CD62E protein, a cell-surface glycoprotein, mediates the adhesion of blood neutrophils to cytokine-activated endothelium through interaction with SELPLG/PSGL1. It also potentially contributes to capillary morphogenesis and interacts with SELPLG/PSGL1 and PODXL2. These multifaceted interactions highlight E-Selectin's role in cell adhesion and its potential influence on vascular development. E-Selectin/CD62E Protein, Mouse (HEK293, His) is the recombinant mouse-derived E-Selectin/CD62E protein, expressed by HEK293 , with C-His, C-10*His labeled tag. The total length of E-Selectin/CD62E Protein, Mouse (HEK293, His) is 536 a.a., with molecular weight of 70-110 kDa.

E-选择素/CD62E 蛋白是一种细胞表面糖蛋白，通过与 SELPLG/PSGL1 相互作用介导血液中性粒细胞与细胞因子激活的内皮细胞的粘附，从而在免疫粘附中发挥至关重要的作用。除了参与免疫反应外，E-选择素还可能有助于毛细血管形态发生。该蛋白质通过唾液酸路易斯 X 表位与 SELPLG/PSGL1 和 PODXL2 相互作用，而这种相互作用似乎不需要 SELPLG 硫酸化。这些相互作用凸显了 E-选择素在介导细胞粘附事件和潜在影响与血管发育相关的过程中的多方面作用。

Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10⁴ cells/well are added to mouse E-Selectin/Fc Chimera coated plates (2 µg/mL with 100 µL/well), approximately 99.43% will adhere for 1 hour incubation at room temperature.

Mouse

HEK293

C-10*His

Q00690/NP_035475.1 (W29-P564)

20339  [NCBI]

WYYNASSELMTYDEASAYCQRDYTHLVAIQNKEEINYLNSNLKHSPSYYWIGIRKVNNVWIWVGTGKPLTEEAQNWAPGEPNNKQRNEDCVEIYIQRTKDSGMWNDERCNKKKLALCYTASCTNASCSGHGECIETINSYTCKCHPGFLGPNCEQAVTCKPQEHPDYGSLNCSHPFGPFSYNSSCSFGCKRGYLPSSMETTVRCTSSGEWSAPAPACHVVECEALTHPAHGIRKCSSNPGSYPWNTTCTFDCVEGYRRVGAQNLQCTSSGIWDNETPSCKAVTCDAIPQPQNGFVSCSHSTAGELAFKSSCNFTCEQSFTLQGPAQVECSAQGQWTPQIPVCKAVQCEALSAPQQGNMKCLPSASGPFQNGSSCEFSCEEGFELKGSRRLQCGPRGEWDSKKPTCSAVKCDDVPRPQNGVMECAHATTGEFTYKSSCAFQCNEGFSLHGSAQLECTSQGKWTQEVPSCQVVQCPSLDVPGKMNMSCSGTAVFGTVCEFTCPDDWTLNGSAVLTCGATGRWSGMPPTCEAPVSPTRP

70-110 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.