EIF5 蛋白, Human (His)

The EIF5 protein is a key member of the 43S pre-initiation complex (43S PIC) and actively participates in mRNA cap-proximal binding, scanning 5'-untranslated regions and locating start codons. EIF5 Protein, Human (GST) is the recombinant human-derived EIF5 protein, expressed by E. coli , with N-GST labeled tag. The total length of EIF5 Protein, Human (GST) is 150 a.a., with molecular weight of 38-43 kDa.

EIF5 是 43S 预起始复合物 (43S PIC) 的重要组成部分，参与蛋白质合成的起始。在这个复合体中，EIF5 在 mRNA 扫描、起始密码子识别和 GTP 水解中发挥着关键作用。EIF5 作为 GTP 酶激活蛋白，促进 eIF2G (EIF2S3) 对 GTP 的水解。在扫描过程中，EIF5 与 EIF1 和 EIF1A 相互作用，有助于维持开放 43S PIC 内的 EIF1。识别起始密码子后，EIF5 诱导 eIF2G (EIF2S3) 水解 GTP，并引发 PIC 构象变化至闭合状态。这一变化增强了 EIF5-CTD 对 EIF2-beta (EIF2S2) 的亲和力，导致 EIF1 从 PIC 中释放。最后，EIF5 将 PIC 稳定在其闭合构象。EIF5 与翻译机制的各个组件相互作用，包括 EIF1A、EIF2-beta (EIF2S2) 和 EIF5B，以及以 RNA 依赖性方式与 FMR1 同工型 6 相互作用，突出显示其在蛋白质合成起始过程中的多方面参与。

Human

E. coli

N-His

P55010 (M1-D150)

1983  [NCBI]

MSVNVNRSVSDQFYRYKMPRLIAKVEGKGNGIKTVIVNMVDVAKALNRPPTYPTKYFGCELGAQTQFDVKNDRYIVNGSHEANKLQDMLDGFIKKFVLCPECENPETDLHVNPKKQTIGNSCKACGYRGMLDTHHKLCTFILKNPPENSD

Approximately 18 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.