Ephrin-B2/EFNB2 蛋白, Human (HEK293, His, Fc)

Ephrin-B2/EFNB2 is a key transmembrane ligand of Eph receptors that coordinates migration, repulsion, and adhesion in developing neurons, blood vessels, and epithelia. It induces bidirectional signaling, binding to EPHA4, EPHA3 and EPHB4, which is critical for cardiac morphogenesis and angiogenesis. Ephrin-B2/EFNB2 Protein, Human (HEK293, His, Fc) is the recombinant human-derived Ephrin-B2/EFNB2 protein, expressed by HEK293, with C-6*His and C-hFc labeled tag.

Ephrin-B2，也被称为 EFNB2，是 Eph 受体的关键细胞表面跨膜配体，Eph 受体是受体酪氨酸激酶家族，对于协调神经元、血管和上皮发育过程中的迁移、排斥和粘附至关重要。Ephrin-B2 显示出与相邻细胞上的 Eph 受体混杂结合的倾向，可引发接触依赖性双向信号传导，分为受体下游的正向信号传导和肝配蛋白配体下游的反向信号传导。其结合亲和力延伸至受体酪氨酸激酶，包括 EPHA4、EPHA3 和 EPHB4，后者在心脏形态发生和血管生成中形成重要的伙伴关系，控制细胞粘附和迁移。在 EPHB4 介导的前向信号传导中，Ephrin-B2 调节细胞排斥和与 EFNB2 表达细胞的分离，可能影响纵向突出轴突的方向。值得注意的是，Ephrin-B2 在微生物感染过程中作为亨德拉病毒和尼帕病毒的受体发挥着独特的作用，为其多方面的功能增添了有趣的一面。

Measured by its binding ability in a functional ELISA. Immobilized Human EPHB2 at 1 μg/mL (100 μL/well) on the plate can bind Biotinylated Human Ephrin-B2. The ED₅₀ for this effect is 2.027 ng/mL.

Human

HEK293

C-6*His;C-hFc

P52799 (I28-A229)

1948

Extracellular Domain

IVLEPIYWNSSNSKFLPGQGLVLYPQIGDKLDIICPKVDSKTVGQYEYYKVYMVDKDQADRCTIKKENTPLLNCAKPDQDIKFTIKFQEFSPNLWGLEFQKNKDYYIISTSNGSLEGLDNQEGGVCQTRAMKILMKVGQDASSAGSTRNKDPTRRPELEAGTNGRSSTTSPFVKPNPGSSTDGNSAGHSGNNILGSEVALFA

Approximately 57-70 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.