1. 重组蛋白
  2. Enzymes & Regulators
  3. Lyases (EC 4)
  4. fbaA 蛋白, Shigella flexneri (His)

fbaA 蛋白, Shigella flexneri (His)

目录号: HY-P701884
产品使用指南

fbaA蛋白通过催化磷酸二羟丙酮(DHAP或磷酸甘油)与3-磷酸甘油醛(G3P)的羟醛缩合,导致形成果糖1,6-二磷酸(FBP)的作用,在细胞代谢中发挥关键作用。这种酶活性对于糖异生和糖酵解至关重要,反映了它参与能量生产和碳通量所需的关键中间体的转化。fbaA 蛋白, Shigella flexneri (His) 是重组的 fbaA 蛋白,由 E. coli 表达,带有 N-6*His 标签。fbaA 蛋白, Shigella flexneri (His) 全长 359 个氨基酸。

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描述

The fbaA protein plays a critical role in cellular metabolism by catalyzing the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerol phosphate) with glyceraldehyde 3-phosphate (G3P), leading to the formation of fructose 1,6-bisphosphate (FBP) role. This enzyme activity is essential for gluconeogenesis and glycolysis, reflecting its involvement in the conversion of key intermediates necessary for energy production and carbon flux. fbaA Protein, Shigella flexneri (His) is the recombinant fbaA protein, expressed by E. coli , with N-6*His labeled tag. The total length of fbaA Protein, Shigella flexneri (His) is 359 a.a., .

研究背景

fbaA 蛋白是一种酶,可催化糖异生和糖酵解中关键的羟醛缩合反应。具体来说,它促进二羟基丙酮磷酸盐(DHAP 或甘油磷酸盐)与甘油醛 3-磷酸盐 (G3P) 的缩合,从而形成果糖 1,6-二磷酸盐 (FBP)。这种可逆反应是糖酵解和糖异生之间代谢物相互转化的核心,在细胞能量代谢中发挥着关键作用。fbaA 酶调节关键磷酸化中间体转化的能力凸显了其在维持细胞内葡萄糖产生和利用平衡方面的重要性。参考文献强调了 fbaA 在这一重要生化途径中的作用,强调了它在细胞能量稳态中的重要性。

种属

Others

表达系统

E. coli

标签

N-6*His

蛋白编号

P0AB73 (M1-L359)

基因 ID

1025905

蛋白结构
N-term
6*His
fbaA (M1-L359)
Accession # P0AB73
C-term
同用名
fbaA; Fructose-bisphosphate aldolase class 2; FBP aldolase; FBPA; Fructose-1; 6-bisphosphate aldolase; Fructose-bisphosphate aldolase class II
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

fbaA Protein, Shigella flexneri (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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fbaA Protein, Shigella flexneri (His)
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