FGF-4 蛋白, Mouse

The FGF-4 protein plays a key role in embryonic development and is central to cell proliferation and differentiation. It is essential for the survival of mouse embryos after implantation and is key to normal limb and heart valve development. FGF-4 Protein, Mouse is the recombinant mouse-derived FGF-4 protein, expressed by E. coli , with tag free. The total length of FGF-4 Protein, Mouse is 136 a.a., with molecular weight of ~15 kDa.

FGF-4 蛋白在胚胎发育过程中发挥着关键作用，是细胞增殖和分化的核心调节因子。它在确保植入后小鼠胚胎存活方面的重要作用强调了它的不可或缺性。在胚胎发生过程中，FGF-4 是协调正常肢体和心脏瓣膜发育的关键角色，反映了其对组织形态发生的多种影响。此外，FGF-4 可能通过诱导牙齿间充质细胞中 MSX1、MSX2 和 SDC1 的表达，参与胚胎磨牙牙芽发育的复杂过程。这种多功能蛋白质与 FGFR1、FGFR2、FGFR3 和 FGFR4 相互作用，形成支持其多方面功能的分子伙伴关系。与硫酸乙酰肝素糖胺聚糖的协同结合增强了 FGF-4 与其受体之间的亲和力，为其调节机制增加了一层复杂性。

Measured in a cell proliferation assay using NIH-3T3 cells. The ED₅₀ for this effect is 0.7284 ng/mL, corresponding to a specific activity is 1.37×10⁶ units/mg.

Mouse

E. coli

Tag Free

P11403 (S67­L202)

14175  [NCBI]

SGAGDYLLGLKRLRRLYCNVGIGFHLQVLPDGRIGGVHADTRDSLLELSPVQRGVVSIFGVASRFFVAMSSRGKLFGVPFFTDECKFKEILLPNNYNAYESYAYPGMFMALSKNGRTKKGNRVSPTMKVTHFLPRL

Approximately 15 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.