FGFR-4 蛋白, Rat (HEK293, C-hFc)

The FGFR-4 protein is a tyrosine-protein kinase receptor for fibroblast growth factor that regulates cellular processes, lipid metabolism, bile acid biosynthesis, and more. Its effects include downregulation of CYP7A1 in response to FGF19. FGFR-4 Protein, Rat (HEK293, C-hFc) is the recombinant rat-derived FGFR-4 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of FGFR-4 Protein, Rat (HEK293, C-hFc) is 351 a.a., with molecular weight of ~110 kDa.

FGFR-4 蛋白是一种酪氨酸蛋白激酶，作为成纤维细胞生长因子的细胞表面受体，在细胞增殖、分化和迁移的调节中发挥着至关重要的作用。此外，它还有助于控制脂质代谢、胆汁酸生物合成、葡萄糖摄取、维生素 D 代谢和磷酸盐稳态。其重要性在 CYP7A1（胆汁酸合成限速酶）响应 FGF19 的正常下调中凸显。配体结合后，FGFR-4 激活各种信号级联反应，包括 PLCG1 和 FRS2 的磷酸化。这种激活导致细胞信号分子的产生，如二酰基甘油和肌醇 1,4,5-三磷酸。此外，FRS2 磷酸化触发 GRB2、GAB1、PIK3R1 和 SOS1 的募集，激活 RAS、MAPK1/ERK2、MAPK3/ERK1、MAP 激酶信号通路和 AKT1 信号通路。值得注意的是，FGFR-4 促进基质蛋白酶 MMP14 的 SRC 依赖性磷酸化，促进其溶酶体降解。为了调节 FGFR-4 信号传导，受体经历内化和降解，这一过程由 MMP14 促进。

1. Measured by its binding ability in a functional ELISA.
2. Immobilized human FGF18 at 10 μg/mL (100 μl/well) can bind Rat FGFR4 , The EC₅₀ of Rat FGFR4 is <1.17 μg/mL.
3.Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH-3T3 mouse fibroblast cells. The ED₅₀  for this effect is 1.025 ng/mL, corresponding to a specific activity is 9.76×10^5 units/mg.

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  Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH-3T3 mouse fibroblast cells. The ED₅₀ for this effect is 1.025 ng/mL, corresponding to a specific activity is 9.76×105 units/mg.

Rat

HEK293

C-hFc

Q498D6 (F17-D367)

25114

FSLEASEEMEQEPCPAPISEQQEQVLTVALGQPVRLCCGRTERGRHWYKEGSRLASAGRVRGWRGRLEIASFLPEDAGRYLCLARGSMTVVHNLTLIMDDSLPSINNEDPKTLSSSSSGHSYLQQAPYWTHPQRMEKKLHAVPAGNTVKFRCPAAGNPMPTIHWLKNGQAFHGENRIGGIRLRHQHWSLVMESVVPSDRGTYTCLVENSLGSIRYSYLLDVLERSPHRPILQAGLPANTTAVVGSNVELLCKVYSDAQPHIQWLKHIVINGSSFGADGFPYVQVLKTTDINSSEVEVLYLRNVSAEDAGEYTCLAGNSIGLSYQSAWLTVLPAEEEDLAWTTATSEARYTD

Approximately 110 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.