FGFR-1 蛋白, Mouse (HEK293, His)

The FGFR-1 protein is a tyrosine-protein kinase receptor for fibroblast growth factors that is critical in embryonic development, cell proliferation, differentiation, and migration. GnRH is essential for mesodermal patterning, axial organization, skeletogenesis, and nervous system development. FGFR-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived FGFR-1 protein, expressed by HEK293 , with C-His labeled tag. The total length of FGFR-1 Protein, Mouse (HEK293, His) is 355 a.a., with molecular weight of 60-80 kDa.

FGFR-1 蛋白是一种酪氨酸蛋白激酶，作为成纤维细胞生长因子的细胞表面受体，在胚胎发育、细胞增殖、分化和迁移的复杂调节中发挥着至关重要的作用。它对于正常中胚层模式、胚胎发育过程中正确的轴向组织、骨骼发生以及促性腺激素释放激素 (GnRH) 神经系统的发育是不可或缺的。配体结合后，FGFR-1 激活多个信号级联，磷酸化 PLCG1、FRS2、GAB1 和 SHB 等关键蛋白。这种激活导致通过 PLCG1 产生信号分子，如二酰基甘油和肌醇 1,4,5-三磷酸。此外，FRS2 的磷酸化会触发 GRB2、GAB1、PIK3R1 和 SOS1 的募集，介导 RAS、MAPK1/ERK2、MAPK3/ERK1、MAP 激酶信号通路和 AKT1 信号通路的激活。FGFR1 还促进 SHC1、STAT1 和 PTPN11/SHP2 的磷酸化。在细胞核内，它增强 RPS6KA1 和 CREB1 的活性，有助于转录调节。FGFR1 信号传导的下调通过 IL17RD/SEF 和 FGFR1 泛素化、内化和降解发生。

Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH 3T3 mouse fibroblast cells. The ED₅₀ for this effect is 28.13ng/mL, corresponding to a specific activity is 35549.2357 Unit/mg.

Mouse

HEK293

C-His

P16092 (R22-E376)

14182  [NCBI]

RPAPTLPEQAQPWGVPVEVESLLVHPGDLLQLRCRLRDDVQSINWLRDGVQLVESNRTRITGEEVEVRDSIPADSGLYACVTSSPSGSDTTYFSVNVSDALPSSEDDDDDDDSSSEEKETDNTKPNRRPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYVQILKTAGVNTTDKEMEVLHLRNVSFEDAGEYTCLAGNSIGLSHHSAWLTVLEALEERPAVMTSPLYLE

Approximately 60-80 kDa due to the glycosylation.

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.