HCLS1 蛋白, Human (HEK293, His)

HCLS1, a substrate of antigen receptor-coupled tyrosine kinase, is pivotal in lymphoid cell antigen receptor signaling, influencing clonal expansion and deletion. Beyond signaling, it may regulate gene expression. HCLS1 forms associations with LCK, LYN, and ANKRD54, exhibiting intricate involvement in signaling networks. Interactions with FES/FPS and FGR add to its functional complexity, emphasizing its role in diverse cellular processes. HCLS1 Protein, Human (HEK293, His) is the recombinant human-derived HCLS1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of HCLS1 Protein, Human (HEK293, His) is 486 a.a., with molecular weight of 55-110 kDa.

HCLS1 是抗原受体偶联酪氨酸激酶的底物，在抗原受体信号传导中发挥着关键作用，影响淋巴细胞的克隆扩增和缺失。除了参与信号级联之外，HCLS1 还可能有助于基因表达的调节。该蛋白与 LCK 的 SH2 和 SH3 结构域形成关联，与 LCK SH3 结构域的结合是组成型的，并且与 LCK SH2 结构域的结合仅在 T 细胞受体 (TCR) 刺激时发生。与 FYN 相比，在 LYN 中观察到类似的结合模式，其中 FYN SH2 区域在 TCR 刺激下结合，而 FYN SH3 区域则不结合，无论 TCR 刺激如何。HCLS1 进一步直接与 HAX1 结合（特别是通过结合至其 C 端区域），并与 HS1BP3 相互作用。值得注意的是，HCLS1 与 LYN 和 ANKRD54 形成多蛋白复合物，强调其在信号网络中的复杂参与。与 FES/FPS 和 FGR 的交互增加了 HCLS1 功能关联的复杂性。

Human

HEK293

C-6*His

P14317 (M1-E486)

3059  [NCBI]

MWKSVVGHDVSVSVETQGDDWDTDPDFVNDISEKEQRWGAKTIEGSGRTEHINIHQLRNKVSEEHDVLRKKEMESGPKASHGYGGRFGVERDRMDKSAVGHEYVAEVEKHSSQTDAAKGFGGKYGVERDRADKSAVGFDYKGEVEKHTSQKDYSRGFGGRYGVEKDKWDKAALGYDYKGETEKHESQRDYAKGFGGQYGIQKDRVDKSAVGFNEMEAPTTAYKKTTPIEAASSGARGLKAKFESMAEEKRKREEEEKAQQVARRQQERKAVTKRSPEAPQPVIAMEEPAVPAPLPKKISSEAWPPVGTPPSSESEPVRTSREHPVPLLPIRQTLPEDNEEPPALPPRTLEGLQVEEEPVYEAEPEPEPEPEPEPENDYEDVEEMDRHEQEDEPEGDYEEVLEPEDSSFSSALAGSSGCPAGAGAGAVALGISAVALYDYQGEGSDELSFDPDDVITDIEMVDEGWWRGRCHGHFGLFPANYVKLLE

55-110 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.