2. 重组蛋白
  3. Enzymes & Regulators
  4. Oxidoreductases (EC 1)
  5. Hygromycin-B kinase/HPH 蛋白, E.coli (His-SUMO)

Hygromycin-B kinase/HPH 蛋白, E.coli (His-SUMO)

目录号: HY-P71517
产品使用指南

潮霉素-B 激酶/HPH 蛋白是一种氨基糖苷类磷酸转移酶，可磷酸化抗生素底物并使其失活，包括潮霉素和相关化合物。这种选择性酶活性突出了其在氨基糖苷类中的特异性，调节抗生素功能并可能影响微生物耐药机制。Hygromycin-B kinase/HPH 蛋白, E.coli (His-SUMO) 是重组的 潮霉素-B 激酶/HPH 蛋白，由 E. coli 表达，带有 N-His, N-SUMO 标签。Hygromycin-B kinase/HPH 蛋白, E.coli (His-SUMO) 全长 341 个氨基酸，分子量约为 ~54.0 kDa。

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描述

Hygromycin-B kinase/HPH Proteinas, an aminoglycoside phosphotransferase, phosphorylates and inactivates antibiotic substrates, including hygromycin and related compounds. This selective enzymatic activity highlights its specificity within the aminoglycoside class, regulating antibiotic function and potentially influencing microbial resistance mechanisms. Hygromycin-B kinase/HPH Protein, E.coli (His-SUMO) is the recombinant E. coli-derived Hygromycin-B kinase/HPH protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of Hygromycin-B kinase/HPH Protein, E.coli (His-SUMO) is 341 a.a., with molecular weight of ~54.0 kDa.
研究背景

潮霉素-B 激酶/HPH 蛋白属于氨基糖苷类磷酸转移酶，通过磷酸化使其抗生素底物失活。具体来说，它选择性地磷酸化潮霉素和密切相关的化合物，包括去甲基类似物和去链霉素。这种酶活性强调了潮霉素-B 激酶/HPH 在修饰氨基糖苷类底物方面的特异性，有助于抗生素功能的调节并可能影响微生物耐药机制。
种属

E.coli
表达系统

E. coli
标签

N-His;N-SUMO
蛋白编号

P00557 (1M-341E)
基因 ID

57286788
蛋白结构
N-term
6*His-SUMO
HPH (1M-341E)
Accession # P00557
C-term
同用名
hphHygromycin-B 4-O-kinase; EC 2.7.1.163; APH(4); Hygromycin B phosphotransferase; Hygromycin-B kinase
氨基酸序列

MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE

分子量

Approximately 54.0 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Hygromycin-B kinase/HPH Protein, E.coli (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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