1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Interferon & Receptors
  4. IFN-γ
  5. IFN-gamma 蛋白, Canine

IFN-gamma 蛋白, Canine

目录号: HY-P79295
COA 产品使用指南

IFN-γ(干扰素-γ)是由 T 细胞和 NK 细胞等免疫细胞产生的 II 型干扰素,在激活抗菌、抗病毒和抗肿瘤反应中发挥关键作用。通过 JAK-STAT 通路,其与受体 IFNGR1 的相互作用触发基因调控。IFN-gamma 蛋白, Canine 是重组的 IFN-γ 蛋白,由 E. coli 表达,不带标签。IFN-gamma 蛋白, Canine 全长 143 个氨基酸,分子量约为 ~16.25 kDa。

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规格 价格 是否有货 数量
Free Sample   Apply now  
5 μg ¥600 In-stock
10 μg ¥1000 In-stock
50 μg ¥2800 In-stock
100 μg ¥4750 In-stock
> 100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

IFN-gamma (interferon-gamma) is a type II interferon produced by immune cells such as T cells and NK cells, and plays a key role in activating antibacterial, antiviral, and antitumor responses. Through the JAK-STAT pathway, its interaction with the receptor IFNGR1 triggers gene regulation. IFN-gamma Protein, Canine is the recombinant canine-derived IFN-gamma protein, expressed by E. coli , with tag free. The total length of IFN-gamma Protein, Canine is 143 a.a., with molecular weight of ~16.25 kDa.

研究背景

IFN-gamma(干扰素-gamma)是一种由 T 细胞和 NK 细胞等免疫细胞产生的 II 型干扰素,通过激活效应免疫细胞和增强抗原呈递,在抗菌、抗病毒和抗肿瘤反应中发挥关键作用。其主要信号通路涉及与其受体 IFNGR1 相互作用的 JAK-STAT 通路,影响基因调控。IFN-γ 结合后,IFNGR1 胞内结构域发生构象变化,促进下游信号成分(包括 JAK2、JAK1 和 STAT1)的关联。该级联导致 STAT1 激活、核转位以及 IFN-γ 调节基因的后续转录,其中许多是像 IRF1 一样的转录因子,能够驱动后续的转录波。IFN-γ 通过诱导免疫蛋白酶体亚基替换催化蛋白酶体亚基,从而促进 I 类抗原呈递途径,从而提高 I 类 MHC 负载的肽的数量、质量和库。它还通过诱导激活剂 PA28 的表达来提高肽生成的效率,PA28 与蛋白酶体结合并改变其蛋白水解切割偏好。此外,IFN-γ 通过促进组织蛋白酶 B/CTSB、H/CTSH 和 L/CTSL 等关键分子的表达,上调细胞表面的 MHC II 复合物。除了其直接的免疫功能外,IFN-γ还参与造血干细胞在发育过程中和稳态条件下的调节,影响其发育、静止和分化。IFN-γ 作为同二聚体存在,通过其胞外结构域与 IFNGR1 相互作用,这是促进 IFNGR1 二聚化的关键相互作用,协调其在免疫反应和造血中的多样化和关键功能。

生物活性

Measured by its ability to inhibit the proliferation of HT-29 human coloncancer cells. The ED50 for this effect is 0.02271 ng/mL, corresponding to a specific activity is 4.40×10^7 Unit/mg.

  • Measured by its ability to inhibit the proliferation of HT-29 human coloncancer cells. The ED50 for this effect is 0.02271 ng/mL, corresponding to a specific activity is 4.40×107 Unit/mg.
种属

Canine

表达系统

E. coli

标签

Tag Free

蛋白编号

P42161 (Q24-K166)

基因 ID
蛋白结构
N-term
IFN-gamma (Q24-K166)
Accession # P42161
C-term
同用名
Interferon gamma; IFN-gamma; IFNG
氨基酸序列

QAMFFKEIENLKEYFNASNPDVSDGGSLFVDILKKWREESDKTIIQSQIVSFYLKLFDNFKDNQIIQRSMDTIKEDMLGKFLNSSTSKREDFLKLIQIPVNDLQVQRKAINELIKVMNDLSPRSNLRKRKRSQNLFRGRRASK

分子量

Approximately 16.25 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

IFN-gamma Protein, Canine 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
IFN-gamma Protein, Canine
目录号:
HY-P79295
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