IGF-I R 蛋白, Mouse (HEK293, His)

The IGF-I R protein, a receptor tyrosine kinase, mediates insulin-like growth factor 1 (IGF1) actions, with high affinity for IGF1. Upon ligand binding, IGF1R activates, leading to autophosphorylation and tyrosine phosphorylation of substrates, including IRS1/2, Shc, and 14-3-3 proteins. Phosphorylation initiates PI3K-AKT/PKB and Ras-MAPK pathways, regulating proliferation and apoptosis. IGF1R signals through JAK/STAT, activating STAT3, contributing to its transforming activity. It also activates JNK and inhibits JNK activation, forming a hybrid receptor with INSR to bind IGF1. IGF-I R Protein, Mouse (HEK293, His) is the recombinant mouse-derived IGF-I R protein, expressed by HEK293, with C-His labeled tag. The total length of IGF-I R Protein, Mouse (HEK293, His) is 906 a.a., with molecular weight of 110-140 & 50-65 kDa, respectively.

IGF-I R 蛋白是一种受体酪氨酸激酶，充当胰岛素样生长因子 1 (IGF1) 作用的介质。它对 IGF1 具有高亲和力，对 IGF2 和胰岛素 (INS) 具有较低亲和力。IGF1R 的激活在控制细胞生长和存活中发挥着关键作用，对肿瘤转化和恶性细胞的存活具有重要影响。配体结合后，受体激酶被激活，导致多种底物的自身磷酸化和酪氨酸磷酸化，包括胰岛素受体底物 (IRS1/2)、Shc 和 14-3-3 蛋白。IRS 蛋白的磷酸化启动两个主要信号传导途径：PI3K-AKT/PKB 途径和 Ras-MAPK 途径。MAPK 途径的激活可增强细胞增殖，而 PI3K 途径的激活可抑制细胞凋亡并刺激蛋白质合成。此外，IGF1R 通过 Janus 激酶/信号转导器和转录激活剂 (JAK/STAT) 发出信号，其中 JAK 蛋白的磷酸化可以激活 STAT 蛋白，特别是 STAT3，从而促进 IGF1R 的转化活性。此外，IGF1R 激活 JNK 激酶并对 JNK 激活发挥抑制作用。当与 INSR 形成杂合受体时，IGF1R 与 IGF1 结合。

1. Immobilized Mouse IGF1R, His Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Human IGFI, hFc Tag with the EC₅₀ of 2.58 μg/mL determined by ELISA.
2.Measured by its binding ability in a functional ELISA. When Recombinant Mouse IGF-I R is immobilized at 5 µg/mL (100 µL/well) can bind Recombinant Human IGF-I. The ED₅₀ for this effect is 322.3 ng/mL.

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  Measured by its binding ability in a functional ELISA. When Recombinant Mouse IGF-I R is immobilized at 5 µg/mL (100 µL/well) can bind Recombinant Human IGF-I. The ED₅₀ for this effect is 322.3 ng/mL.

Mouse

HEK293

C-His

Q60751-1 (E31-H936)

16001  [NCBI]

EICGPGIDIRNDYQQLKRLENCTVIEGFLHILLISKAEDYRSYRFPKLTVITEYLLLFRVAGLESLGDLFPNLTVIRGWKLFYNYALVIFEMTNLKDIGLYNLRNITRGAIRIEKNADLCYLSTIDWSLILDAVSNNYIVGNKPPKECGDLCPGTLEEKPMCEKTTINNEYNYRCWTTNRCQKMCPSVCGKRACTENNECCHPECLGSCHTPDDNTTCVACRHYYYKGVCVPACPPGTYRFEGWRCVDRDFCANIPNAESSDSDGFVIHDDECMQECPSGFIRNSTQSMYCIPCEGPCPKVCGDEEKKTKTIDSVTSAQMLQGCTILKGNLLINIRRGNNIASELENFMGLIEVVTGYVKIRHSHALVSLSFLKNLRLILGEEQLEGNYSFYVLDNQNLQQLWDWNHRNLTVRSGKMYFAFNPKLCVSEIYRMEEVTGTKGRQSKGDINTRNNGERASCESDVLRFTSTTTWKNRIIITWHRYRPPDYRDLISFTVYYKEAPFKNVTEYDGQDACGSNSWNMVDVDLPPNKEGEPGILLHGLKPWTQYAVYVKAVTLTMVENDHIRGAKSEILYIRTNASVPSIPLDVLSASNSSSQLIVKWNPPTLPNGNLSYYIVRWQRQPQDGYLYRHNYCSKDKIPIRKYADGTIDVEEVTENPKTEVCGGDKGPCCACPKTEAEKQAEKEEAEYRKVFENFLHNSIFVPRPERRRRDVMQVANTTMSSRSRNTTVADTYNITDPEEFETEYPFFESRVDNKERTVISNLRPFTLYRIDIHSCNHEAEKLGCSASNFVFARTMPAEGADDIPGPVTWEPRPENSIFLKWPEPENPNGLILMYEIKYGSQVEDQRECVSRQEYRKYGGAKLNRLNPGNYTARIQATSLSGNGSWTDPVFFYVPAKTTYENFMH

110-140 & 50-65 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.