IL-17F, Rat (sf9, His)

Rat IL-17F is predicted to have cytokine binding and dimerization activities and is involved in bacterial defense, IL-17-mediated signaling, and regulation of cytokine production. It acts upstream of the inflammatory response, and its homologue to human IL17F has been implicated in chronic mucocutaneous candidiasis. IL-17F, Rat (sf9, His) is the recombinant rat-derived IL-17F, Rat, expressed by E. coli , with N-6*His labeled tag.

IL-17F，大鼠预计具有细胞因子结合活性、蛋白质二聚化活性和信号受体结合活性。该基因预计参与多种过程，包括对细菌的防御反应、白细胞介素 17 介导的信号通路以及细胞因子产生的调节。预计它在参与炎症反应的细胞因子产生的上游或正向调节范围内发挥作用，并在 RNA 聚合酶 II 的转录正向调节中发挥作用。预期的亚细胞位置是在细胞外空间。作为人 IL17F（白细胞介素 17F）的直系同源物，大鼠 IL-17F 与慢性皮肤粘膜念珠菌病有关。尽管其功能重要，但在参考数据集中观察到低表达水平，表明其调节功能可能受到严格控制或依赖于上下文。

Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 45.19 ng/mL, corresponding to a specific activity is 2.212×10⁴ U/mg.

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  Measured by its ability to induce IL-6 secretion by NIH-3T3 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 45.19 ng/mL, corresponding to a specific activity is 2.212×10⁴ U/mg.

Rat

E. coli

N-6*His

NP_001015011.2 (A28-A161)

301291  [NCBI]

Full Length of Mature Protein

ARRNPKVGLSALQKAGNCPPLEDNSVRVDIRIFNQNQGISVPRDFQNRSSSPWDYNITRDPDRFPSEIAEAQCRHSGCINAQGQEDGSMNSVPIQQEILVLRREPQGCSNSFRLEKMLIKVGCTCVTPIVHHAA

Approximately 18 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 4 mM HCL, pH 2.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.