1. 重组蛋白
  2. Cytokines and Growth Factors
  3. CSF & Receptors
  4. Multi-CSF/IL-3
  5. IL-3 蛋白, Rhesus macaque (His)

IL-3 蛋白, Rhesus macaque (His)

目录号: HY-P71858A
COA 产品使用指南

IL-3蛋白是一种造血细胞因子,通过影响各种白细胞群的产生、分化和功能来调节造血功能。它充当集落刺激因子,促进这些细胞类型的发育和成熟并维持功能性免疫系统。IL-3 蛋白, Rhesus macaque (His) 是重组的 IL-3 蛋白,由 E. coli 表达,带有 N-6*His 标签。IL-3 蛋白, Rhesus macaque (His) 全长 124 个氨基酸,分子量约为 approximately 17 kDa。

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规格 价格 是否有货 数量
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2 μg ¥640 In-stock
10 μg ¥1790 In-stock
50 μg ¥5000 In-stock
100 μg ¥8500 In-stock
> 100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

IL-3 protein, a hematopoietic cytokine, regulates hematopoiesis by influencing the production, differentiation, and function of various white blood cell populations. It acts as a colony-stimulating factor, promoting the development and maturation of these cell types and maintaining a functional immune system. IL-3 Protein, Rhesus macaque (His) is the recombinant Rhesus Macaque-derived IL-3 protein, expressed by E. coli , with N-6*His labeled tag. The total length of IL-3 Protein, Rhesus macaque (His) is 124 a.a., with molecular weight of approximately 17 kDa.

研究背景

IL-3 是一种造血细胞因子,通过影响不同白细胞群(包括粒细胞、单核细胞、巨噬细胞、肥大细胞、干细胞、红细胞、嗜酸性粒细胞和巨核细胞)的产生、分化和功能来对造血进行调节控制。作为集落刺激因子,IL-3 在促进血液中各种细胞类型的发育和成熟方面发挥着至关重要的作用,有助于维持功能性和平衡的免疫系统。

生物活性

Measured in a cell proliferation assay using using TF1 human human erythroleukemic cells.The ED50 for this effect is 0.8725 ng/mL, corresponding to a specific activity is 1.146×106 units/mg.

  • Measured in a cell proliferation assay using TF1 human erythroleukemic cells.The ED50 for this effect is 0.8725 ng/mL, corresponding to a specific activity is 1.146×106 units/mg.
种属

Rhesus Macaque

表达系统

E. coli

标签

N-6*His

蛋白编号

P25140 (A20-Q143)

基因 ID
蛋白结构
N-term
6*His
IL-3 (A20-Q143)
Accession # P25140
C-term
同用名
IL3; Interleukin-3; IL-3; Hematopoietic growth factor; Mast cell growth factor; MCGF; Multipotential colony-stimulating factor; P-cell-stimulating factor
氨基酸序列

APMTQTTSLKTSWAKCSNMIDEIITHLNQPPLPSPDFNNLNEEDQTILVEKNLRRSNLEAFSKAVKSLQNASAIESILKNLPPCLPMATAAPTRPPIRITNGDRNDFRRKLKFYLKTLENEQAQ

分子量

approximately 17 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

IL-3 Protein, Rhesus macaque (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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