IL-37 蛋白, Human

IL-37 protein is an important immunoregulatory cytokine that suppresses innate inflammation and immune responses and reduces excessive inflammation. It signals intracellularly through nuclear translocation of SMAD3 and extracellularly through binding to its receptors, consisting of IL18R1 and IL18RAP. IL-37 Protein, Human is the recombinant human-derived IL-37 protein, expressed by E. coli , with tag free. The total length of IL-37 Protein, Human is 166 a.a., with molecular weight of ~18-20 kDa.

IL-37 蛋白是一种免疫调节细胞因子，作为先天炎症和免疫反应的抑制剂发挥着关键作用，可减轻过度炎症。信号传导通过两种不同的机制发生：细胞内通过 SMAD3 的核转位，以及分泌并与其受体（由 IL18R1 和 IL18RAP 组成）结合后在细胞外发生。IL-37 抑制或减少促炎细胞因子的产生，包括 IL1A、IL6、CCL12、CSF1、CSF2、CXCL13、IL1B、IL23A 和 IL1RN，同时保留抗炎细胞因子。它还抑制树突状细胞活化。IL-37 与 SMAD3 相互作用并结合 IL18R1，尽管亲和力低于 IL18，但不充当 IL18 受体拮抗剂。此外，它与货物受体 TMED10 形成复合物，促进从细胞质易位到内质网-高尔基中间室 (ERGIC) 和随后的分泌。

Immobilized Recombinant human IL-18 R alpha /IL-1 R5 at 2 μg/mL (100 μL/well) can bind Biotinylated Recombinant human IL-37 Protein. The ED₅₀ for this effect is <512 ng/mL.

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  Immobilized Recombinant human IL-18 R alpha /IL-1 R5 at 2 μg/mL (100 μL/well) can bind Biotinylated Recombinant human IL-37 Protein. The ED₅₀ for this effect is 484.2 ng/mL.

Human

E. coli

Tag Free

Q9NZH6 (K53-D218)

27178  [NCBI]

KNLNPKKFSIHDQDHKVLVLDSGNLIAVPDKNYIRPEIFFALASSLSSASAEKGSPILLGVSKGEFCLYCDKDKGQSHPSLQLKKEKLMKLAAQKESARRPFIFYRAQVGSWNMLESAAHPGWFICTSCNCNEPVGVTDKFENRKHIEFSFQPVCKAEMSPSEVSD

Approximately 18-20 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 2 mM DTT, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Lijuan Wang, et al. Interleukin-37: A crucial cytokine with multiple roles in disease and potentially clinical therapy. Oncol Lett. 2018 Apr;15(4):4711-4719.  [Content Brief]