Integrin alpha 5 beta 1 蛋白, Human (HEK293, Flag-His)

ITGA5, a versatile receptor, interacts with diverse ligands such as fibronectin, fibrinogen, PLA2G2A, fibrillin-1 (FBN1), IL1B, Human metapneumovirus, and soluble CD40LG. Recognizing the R-G-D sequence, ITGA5 mediates cell adhesion through distinct binding sites. Notably, it binds PLA2G2A at a separate site, inducing conformational changes enhancing ligand binding. ITGA5's role in IL1B signaling, microbial infection, and CD40/CD40LG signaling highlights its multifunctionality in cellular processes and signaling pathways. Integrin alpha 5 beta 1 Protein, Human (HEK293, Flag-His) is a recombinant protein dimer complex containing human-derived Integrin alpha 5 beta 1 protein, expressed by HEK293, with C-Flag, C-10*His labeled tag. Integrin alpha 5 beta 1 Protein, Human (HEK293, Flag-His), has molecular weight of ~101.35 kDa.

整合素 α-5/β-1 蛋白 (ITGA5:ITGB1) 作为具有多种配体相互作用的多功能受体。ITGA5:ITGB1 识别其配体中的 RGD 序列，充当纤连蛋白和纤维蛋白原的受体，通过不同的结合位点介导细胞粘附。值得注意的是，它在与其经典配体结合位点不同的位点上与 PLA2G2A 结合，从而诱导增强配体结合的构象变化。此外，ITGA5:ITGB1 作为 fibrillin-1 (FBN1) 的受体，促进 RGD 依赖性细胞粘附。它也是纤连蛋白 (FN1) 的受体，使 RGD 依赖性细胞粘附到 FN1。此外，ITGA5:ITGB1 作为 IL1B 受体，在 IL1B 信号传导中发挥着至关重要的作用。在微生物感染的情况下，ITGA5:ITGB1 作为人类偏肺病毒的受体，强调了它参与病原体识别。此外，ITGA5:ITGB3 作为可溶性 CD40LG 的受体，在 CD40/CD40LG 信号传导中发挥着至关重要的作用。这种广泛的配体相互作用强调了 ITGA5:ITGB1 在细胞过程和信号通路中的多功能性。

Immobilized Fibronectin at 2 μg/mL (100 μL/well) can bind Biotinylated Integrin alpha 5 beta 1 protein. The ED₅₀ for this effect is 2.49 ng/mL, corresponding to a specific activity is 4.02×10^5 Unit/mg.

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  Immobilized Fibronectin at 2 μg/mL (100 μL/well) can bind Biotinylated Integrin alpha 5 beta 1 protein. The ED₅₀ for this effect is 2.49 ng/mL, corresponding to a specific activity is 4.02×10⁵ Unit/mg.

Human

HEK293

C-Flag;C-10*His

P08648 (F42-Y995) & P05556-1 (Q21-D728)

3678  [NCBI]&3688  [NCBI]

FNLDAEAPAVLSGPPGSFFGFSVEFYRPGTDGVSVLVGAPKANTSQPGVLQGGAVYLCPWGASPTQCTPIEFDSKGSRLLESSLSSSEGEEPVEYKSLQWFGATVRAHGSSILACAPLYSWRTEKEPLSDPVGTCYLSTDNFTRILEYAPCRSDFSWAAGQGYCQGGFSAEFTKTGRVVLGGPGSYFWQGQILSATQEQIAESYYPEYLINLVQGQLQTRQASSIYDDSYLGYSVAVGEFSGDDTEDFVAGVPKGNLTYGYVTILNGSDIRSLYNFSGEQMASYFGYAVAATDVNGDGLDDLLVGAPLLMDRTPDGRPQEVGRVYVYLQHPAGIEPTPTLTLTGHDEFGRFGSSLTPLGDLDQDGYNDVAIGAPFGGETQQGVVFVFPGGPGGLGSKPSQVLQPLWAASHTPDFFGSALRGGRDLDGNGYPDLIVGSFGVDKAVVYRGRPIVSASASLTIFPAMFNPEERSCSLEGNPVACINLSFCLNASGKHVADSIGFTVELQLDWQKQKGGVRRALFLASRQATLTQTLLIQNGAREDCREMKIYLRNESEFRDKLSPIHIALNFSLDPQAPVDSHGLRPALHYQSKSRIEDKAQILLDCGEDNICVPDLQLEVFGEQNHVYLGDKNALNLTFHAQNVGEGGAYEAELRVTAPPEAEYSGLVRHPGNFSSLSCDYFAVNQSRLLVCDLGNPMKAGASLWGGLRFTVPHLRDTKKTIQFDFQILSKNLNNSQSDVVSFRLSVEAQAQVTLNGVSKPEAVLFPVSDWHPRDQPQKEEDLGPAVHHVYELINQGPSSISQGVLELSCPQALEGQQLLYVTRVTGLNCTTNHPINPKGLELDPEGSLHHQQKREAPSRSSASSGPQILKCPEAECFRLRCELGPLHQQESQSLQLHFRVWAKTFLQREHQPFSLQCEAVYKALKMPYRILPRQLPQKERQVATAVQWTKAEGSY&QTDENRCLKANAKSCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDLEALKKKGCPPDDIENPRGSKDIKKNKNVTNRSKGTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFNELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNMYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSANSSNVIQLIIDAYNSLSSEVILENGKLSEGVTISYKSYCKNGVNGTGENGRKCSNISIGDEVQFEISITSNKCPKKDSDSFKIRPLGFTEEVEVILQYICECECQSEGIPESPKCHEGNGTFECGACRCNEGRVGRHCECSTDEVNSEDMDAYCRKENSSEICSNNGECVCGQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLICGGNGVCKCRVCECNPNYTGSACDCSLDTSTCEASNGQICNGRGICECGVCKCTDPKFQGQTCEMCQTCLGVCAEHKECVQCRAFNKGEKKDTCTQECSYFNITKVESRDKLPQPVQPDPVSHCKEKDVDDCWFYFTYSVNGNNEVMVHVVENPECPTGPD

Approximately 101.35 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.