Jagged-1/JAG1 蛋白, Rat (HEK293, His)

Jagged-1/JAG1 protein is a ligand of multiple Notch receptors, mediates Notch signaling, affects cell fate decisions during hematopoiesis and enhances angiogenesis. It functions throughout mammalian cardiovascular development, regulates myoblast differentiation, and participates in the complex Notch signaling network through interactions with NOTCH1, NOTCH2, and NOTCH3. Jagged-1/JAG1 Protein, Rat (HEK293, His) is the recombinant rat-derived Jagged-1/JAG1 protein, expressed by HEK293.

Jagged-1 (JAG1) 作为多个 Notch 受体的配体，在介导 Notch 信号传导中发挥着关键作用。它与造血过程中的细胞命运决定有关，并显示出在体外增强成纤维细胞生长因子诱导的血管生成的能力。JAG1 的参与跨越了哺乳动物心血管发育的各个阶段，凸显了它在这些过程中的重要性。此外，它对成肌细胞分化具有抑制作用，表明其在多种细胞功能中的调节作用。通过与 NOTCH1、NOTCH2 和 NOTCH3 的相互作用，JAG1 有助于形成复杂的 Notch 信号传导网络，强调其在细胞反应和发育途径中的多方面作用。

Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 1.264 μg/mL, corresponding to a specific activity is 791.1392 units/mg.

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  Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 1.264 μg/mL, corresponding to a specific activity is 791.1392 units/mg.

Rat

HEK293

C-6*His

Q63722 (S32-D1067, with substitutions GGARN56-60AE and DR63-64TLVRPY)

29146

Partial

Approximately 125-130 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

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  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.