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  3. Jagged-2/JAG2 蛋白, Human (CHO, hFc)

Jagged-2/JAG2 蛋白, Human (CHO, hFc)

目录号: HY-P79094
COA 产品使用指南 技术支持

JAG2 是 NOTCH 配体之一。JAG2 通过 p21 调节 CRC 细胞对化疗药物的敏感性。JAG2 参与独立于经典 Notch 信号通路的迁移和侵袭的调节。JAG2还以非EMT途径促进结肠癌细胞的迁移和侵袭。Jagged-2/JAG2 蛋白, Human (CHO, hFc) 是重组的 Jagged-2/JAG2 蛋白,由 CHO 表达,带有 C-hFc 标签。

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规格 价格 是否有货 数量
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5 μg ¥1200 In-stock
10 μg ¥1900 In-stock
50 μg ¥3400 In-stock
100 μg ¥5400 In-stock
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

JAG2 is one of the NOTCH ligands. JAG2 modulates the sensitivity of CRC cells to chemotherapeutic agents through p21. JAG2 was involved in the regulation of migration and invasion independent of the canonical Notch signaling pathway. JAG2 also promoted the migration and invasion of colon cancer cells in a non-EMT pathway[1][2]. Jagged-2/JAG2 Protein, Human (CHO, hFc) is the recombinant human-derived Jagged-2/JAG2 protein, expressed by CHO , with C-hFc labeled tag.

研究背景

JAG2表达的调节显着影响CRC细胞系的体内致瘤性。此外,JAG2的敲低使CRC细胞对化疗药物敏感,而JAG2的异位表达增加了CRC细胞的化疗耐药性。在 JAG2 敲低细胞中观察到 p21 显着下调。NOTCH 信号传导由四个受体 (NOTCH1-NOTCH4)、三个 delta 样配体(DLL1、DLL3 和 DLL4) 和两个锯齿状配体 (JAG1 和 JAG2) 组成。β-连环蛋白的敲低或药理学抑制可降低 CRC 细胞系中的 JAG2 水平[1]。人类JAG2基因位于染色体14q32,由26个外显子组成,编码长度为5825bp和5721bp的mRNA变体。该基因编码的蛋白质包含一个信号肽、一个胞外DSL区域、16个EGF样重复序列、一个富含半胱氨酸的区域、一个跨膜区域和一个由132个氨基酸组成的短胞质区域[2]

生物活性

Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 0.5-2 µg/mL.

种属

Human

表达系统

CHO

标签

C-hFc

蛋白编号

AAB84215 (M27-L1082)

基因 ID
蛋白结构
N-term
JAG2 (M27-L1082)
Accession # AAB84215
hFc
C-term
中文名
Jagged-2 蛋白/JAG2 蛋白
同用名
Protein jagged-2; JAG2; Jagged2; hJ2
预测分子量
138.8 kDa
分子量

Approximately 140 kDa, based on SDS-PAGE under reducing conditions.

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末.

组分

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 1mM EDTA.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Jagged-2/JAG2 Protein, Human (CHO, hFc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Jagged-2/JAG2 Protein, Human (CHO, hFc)
目录号:
HY-P79094
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