Jagged-2/JAG2 蛋白, Human (CHO, hFc)

JAG2 is one of the NOTCH ligands. JAG2 modulates the sensitivity of CRC cells to chemotherapeutic agents through p21. JAG2 was involved in the regulation of migration and invasion independent of the canonical Notch signaling pathway. JAG2 also promoted the migration and invasion of colon cancer cells in a non-EMT pathway^[1][2]. Jagged-2/JAG2 Protein, Human (CHO, hFc) is the recombinant human-derived Jagged-2/JAG2 protein, expressed by CHO , with C-hFc labeled tag.

JAG2表达的调节显着影响CRC细胞系的体内致瘤性。此外，JAG2的敲低使CRC细胞对化疗药物敏感，而JAG2的异位表达增加了CRC细胞的化疗耐药性。在 JAG2 敲低细胞中观察到 p21 显着下调。NOTCH 信号传导由四个受体 (NOTCH1-NOTCH4)、三个 delta 样配体(DLL1、DLL3 和 DLL4) 和两个锯齿状配体 (JAG1 和 JAG2) 组成。β-连环蛋白的敲低或药理学抑制可降低 CRC 细胞系中的 JAG2 水平^[1]。人类JAG2基因位于染色体14q32，由26个外显子组成，编码长度为5825bp和5721bp的mRNA变体。该基因编码的蛋白质包含一个信号肽、一个胞外DSL区域、16个EGF样重复序列、一个富含半胱氨酸的区域、一个跨膜区域和一个由132个氨基酸组成的短胞质区域^[2]。

Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is 0.5-2 µg/mL.

Human

CHO

C-hFc

AAB84215 (M27-L1082)

3714  [NCBI]

Approximately 140 kDa, based on SDS-PAGE under reducing conditions.

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 1mM EDTA.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.