1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. JMJD2D 蛋白, Human

JMJD2D 蛋白, Human

目录号: HY-P701620
产品使用指南

JMJD2D 蛋白是复杂组蛋白密码中的核心组蛋白去甲基化酶,专门针对组蛋白 H3 的“Lys-9”。重要的是,它不会使 H3“Lys-4”、“Lys-27”、“Lys-36”或 H4“Lys-20”去甲基化。JMJD2D 蛋白, Human 是重组的 JMJD2D 蛋白,由 E. coli 表达,不带标签。JMJD2D 蛋白, Human 全长 522 个氨基酸。

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     可免费申领三个不同产品的试用装。

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描述

The JMJD2D protein is a core histone demethylase in the complex histone code, specifically targeting "Lys-9" of histone H3. Importantly, it does not demethylate H3 "Lys-4", "Lys-27", "Lys-36" or H4 "Lys-20". JMJD2D Protein, Human is the recombinant human-derived JMJD2D protein, expressed by E. coli , with tag free. The total length of JMJD2D Protein, Human is 522 a.a., .

研究背景

JMJD2D 蛋白是一种组蛋白去甲基化酶,通过特异性靶向组蛋白 H3 的“Lys-9”,在复杂的组蛋白密码中发挥核心作用。值得注意的是,JMJD2D 不会使组蛋白 H3“Lys-4”、H3“Lys-27”、H3“Lys-36”或 H4“Lys-20”去甲基化。其去甲基酶活性仅限于二甲基化和三甲基化 H3 'Lys-9' 残基,对单甲基化残基没有明显影响。JMJD2D 催化的去甲基化过程导致甲醛和琥珀酸的生成。这种特定的去甲基化模式强调了 JMJD2D 在塑造表观遗传景观中的重要性,揭示了其在组蛋白修饰动态调节中的独特作用。

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

Q6B0I6 (E2-P523)

基因 ID

55693

蛋白结构
N-term
JMJD2D (E2-P523)
Accession # Q6B0I6
C-term
同用名
KDM4D; Lysine-specific demethylase 4D; JmjC domain-containing histone demethylation protein 3D; Jumonji domain-containing protein 2D; [histone H3]-trimethyl-L-lysine(9) demethylase 4D
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

JMJD2D Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
JMJD2D Protein, Human
目录号:
HY-P701620
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