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  3. LRRFIP1 蛋白, Human (His, Strep)

LRRFIP1 蛋白, Human (His, Strep)

目录号: HY-P702001
产品使用指南

LRRFIP1 是一种转录抑制因子,优先与富含 GC 的序列 (5'-AGCCCCCGGCG-3') 结合,并可能调节 TNF、EGFR 和 PDGFA 的表达。它抑制 PDGFA,后者控制动脉损伤后的平滑肌细胞增殖。LRRFIP1 蛋白, Human (His, Strep) 是重组的 LRRFIP1 蛋白,由 E. coli 表达,带有 N-Strep, N-6*His 标签。LRRFIP1 蛋白, Human (His, Strep) 全长 808 个氨基酸。

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描述

LRRFIP1 is a transcriptional repressor that preferentially binds to GC-rich sequences (5'-AGCCCCCGGCG-3') and may regulate the expression of TNF, EGFR, and PDGFA. It inhibits PDGFA, which controls smooth muscle cell proliferation after arterial injury. LRRFIP1 Protein, Human (His, Strep) is the recombinant human-derived LRRFIP1 protein, expressed by E. coli , with N-Strep, N-6*His labeled tag. The total length of LRRFIP1 Protein, Human (His, Strep) is 808 a.a., .

研究背景

LRRFIP1 作为转录阻遏蛋白,优先结合富含 GC 的共有序列 (5'-AGCCCCCGGCG-3'),并参与 TNF、EGFR 和 PDGFA 表达的潜在调节。这种调节作用延伸到动脉损伤后平滑肌细胞增殖的控制,这是通过抑制 PDGFA 来实现的。此外,LRRFIP1 表现出结合双链 RNA 的能力。它在响应激动剂的 Toll 样受体 (TLR) 信号传导中发挥积极作用,可能是通过与 MYD88 结合的负 FLII 调节因子竞争性相互作用。作为同二聚体存在,LRRFIP1 也可以形成更高阶的寡聚体,并且它与 FLII 和 MYD88 相互作用,与 FLII 竞争 MyD88 结合,即使在没有脂多糖 (LPS) 的情况下也是如此。

种属

Human

表达系统

E. coli

标签

N-Strep;N-6*His;C-Flag

蛋白编号

Q32MZ4 (M1-S808)

基因 ID

9208

蛋白结构
N-term
Strep
6*His
LRRFIP1 (M1-S808)
Accession # Q32MZ4
Flag
C-term
同用名
LRRFIP1; Leucine-rich repeat flightless-interacting protein 1; LRR FLII-interacting protein 1; GC-binding factor 2; TAR RNA-interacting protein
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

LRRFIP1 Protein, Human (His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
LRRFIP1 Protein, Human (His, Strep)
目录号:
HY-P702001
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