MCP-2/CCL8 蛋白, Mouse

MCP-2/CCL8 Protein, Mouse is a CC chemokine that interacts with CCR1, CCR2B, CCR3, and CCR5 to mediate host inflammatory immune responses, tumorigenesis, and antiviral infections. MCP-2/CCL8 Protein, Mouse is a recombinant mouse MCP-2/CCL8 (G24-P97) protein expressed by E. coli^[1][2].

CCL8，也称为单核细胞趋化蛋白 2 (MCP2)，是属于 CC 趋化因子家族的小细胞因子。它首先在人骨肉瘤细胞中发现，是由位于人17号染色体上的CCL8基因编码的蛋白质。CCL8主要在小肠和外周血细胞中表达。CCL8 可以结合多种不同的趋化因子细胞表面受体，例如 CCR1、CCR2B、CCR3 和 CCR5。CCL8可以充当化学引诱剂，吸引单核细胞、淋巴细胞、嗜碱性粒细胞和嗜酸性粒细胞等趋化因子来介导炎症宿主反应。一方面，CCL8有助于乳腺癌的扩散，促进食管鳞癌的迁移和侵袭。另一方面，也有报道称CCL8抑制宫颈癌肿瘤生长，并在黑色素瘤中表现出抗肿瘤转移作用。cCL8 显着激活胶质母细胞瘤细胞中的 ERK1/2 磷酸化，并通过中和 tama 分泌的 CCL8 的抗体阻断来显着降低胶质瘤细胞的侵袭性。同时，CCL8 可以作为一种有效的 HIV1 抑制剂，对受体 CCR5 具有高亲和力，CCR5 是 HIV1 的主要共同受体之一^[1][2]。

MCP2/CCL8 (20-180 µg/mL) can stimulate the proliferation of splenic lymphocytes of immunosuppressed mice in a dose-dependent manner, with the highest proliferation index at a concentration of 180 μg/mL, which is 3.82 times higher than that of the normal group^[3].

MCP2/CCL8(i.p., 1 μg in 200 μL of PBS, once daily, 14 days) decreases in the number and size of tumor colonies in the liver compared with vector-treated mice, suggesting an anti-tumor metastatic effect in C57BL/6 mice with B16 F10 cells^[4].

1. Full biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/ml.
2. Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED₅₀ this effect is 0.05265 μg/mL, corresponding to a specific activity is 1.899×10^4 U/mg.

• 
  Measured by its ability to chemoattract THP-1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 0.05265 μg/mL,corresponding to a specific activity is 1.899×10⁴ U/mg.

Mouse

E. coli

Tag Free

Q9Z121 (G24-P97)

20307  [NCBI]

GPDKAPVTCCFHVLKLKIPLRVLKSYERINNIQCPMEAVVFQTKQGMSLCVDPTQKWVSEYMEILDQKSQILQP

Approximately 10.54 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against 20 mM PB, pH 7.4, 150 mM NaCl or 20 mM PB, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Xiang Zhang, et al. CCL8 secreted by tumor-associated macrophages promotes invasion and stemness of glioblastoma cells via ERK1/2 signaling. Lab Invest. 2020 Apr;100(4):619-629.  [Content Brief]

  [2]. Jan Korbecki, et al. CC Chemokines in a Tumor: A Review of Pro-Cancer and Anti-Cancer Properties of the Ligands of Receptors CCR1, CCR2, CCR3, and CCR4. Int J Mol Sci. 2020 Nov 9;21(21):8412.  [Content Brief]

  [3]. Yuan-Yuan Deng, et al. Immunomodulatory activity and partial characterisation of polysaccharides from Momordica charantia. Molecules. 2014 Aug 29;19(9):13432-47.  [Content Brief]

  [4]. Kiyokazu Hiwatashi, et al. Suppression of SOCS3 in macrophages prevents cancer metastasis by modifying macrophage phase and MCP2/CCL8 induction. Cancer Lett. 2011 Sep 28;308(2):172-80.  [Content Brief]