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  4. MetC 蛋白, E.coli (E. coli, His)

MetC 蛋白, E.coli (E. coli, His)

目录号: HY-P71694
产品使用指南

MetC 蛋白是一种多功能酶，可催化胱硫醚裂解为高半胱氨酸、丙酮酸和氨，这对于蛋氨酸生物合成至关重要。它还充当半胱氨酸脱硫酶，将半胱氨酸转化为硫化物。MetC 蛋白, E.coli (E. coli, His) 是重组的 MetC 蛋白，由 E. coli 表达，带有 N-His 标签。

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规格 是否有货
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100 μg   询价  

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描述

MetC protein is a multifunctional enzyme that catalyzes the cleavage of cystathionine to homocysteine, pyruvate, and ammonia, which is essential for methionine biosynthesis. It also acts as a cysteine desulfurase, converting cysteine into sulfide. MetC Protein, E.coli (E. coli, His) is the recombinant E. coli-derived MetC protein, expressed by E. coli , with N-His labeled tag.
研究背景

MetC 蛋白是一种多功能酶，主要作用是催化胱硫醚裂解为同型半胱氨酸、丙酮酸和氨，这是蛋氨酸生物合成的关键步骤。除了其主要功能外，MetC 还具有半胱氨酸脱硫酶活性，可将半胱氨酸转化为硫化物。此外，在特定的生长条件下，该蛋白质作为丙氨酸消旋酶表现出显着的共活性。这种多功能酶库强调了 MetC 参与多种代谢途径，展示了其对氨基酸代谢和生物合成过程的复杂调节做出贡献的能力。
种属

E.coli
表达系统

E. coli
标签

N-His
蛋白编号

P06721 (M1-A392)
基因 ID

946240  [NCBI]
蛋白结构
N-term
His
MetC (M1-A392)
Accession # P06721
C-term
同用名
metC; b3008; JW2975Cystathionine beta-lyase MetC; CBL; CL; CD; EC 4.4.1.28; Cysteine lyase MetC; Cysteine-S-conjugate beta-lyase MetC
氨基酸序列

MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKHATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFIEQGDHVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSITMEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDAMIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEWLAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNNEELANYLDNFSLFSMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA

分子量

Approximately 46.8 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

MetC Protein, E.coli (E. coli, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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