PGD2 Synthase/PTGDS 蛋白, Rat (HEK293, His)

The PGD2 Synthase, also known as PTGDS protein, is a key enzymatic player in diverse physiological processes. It catalyzes the conversion of PGH2 to PGD2, a prostaglandin essential for smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Beyond its vascular functions, PTGDS is intricately involved in various central nervous system (CNS) activities, including sedation, NREM sleep, and PGE2-induced allodynia, and may exert an anti-apoptotic role in oligodendrocytes. The protein exhibits a broad substrate-binding capacity, interacting with small non-substrate lipophilic molecules like biliverdin, bilirubin, retinal, retinoic acid, and thyroid hormone, potentially acting as a scavenger for harmful hydrophobic molecules and functioning as a secretory retinoid and thyroid hormone transporter. PTGDS is implicated in the development and maintenance of blood barriers, including the blood-brain, blood-retina, blood-aqueous humor, and blood-testis barriers, highlighting its significance in barrier function. Moreover, it plays pivotal roles in both the maturation and maintenance of the central nervous system and the male reproductive system. Additionally, PTGDS participates in PLA2G3-dependent mast cell maturation, where PLA2G3, secreted by immature mast cells, acts upstream to PTGDS to synthesize PGD2. This, in turn, promotes mast cell maturation and degranulation via PTGDR, indicating its involvement in immune responses and cellular maturation. PGD2 Synthase/PTGDS Protein, Rat (HEK293, His) is the recombinant rat-derived PGD2 Synthase/PTGDS protein, expressed by HEK293 , with C-His, C-6*His labeled tag. The total length of PGD2 Synthase/PTGDS Protein, Rat (HEK293, His) is 165 a.a., with molecular weight of ~26-29 kDa.

PGD2 合酶，也称为 PTGDS 蛋白，是多种生理过程中的关键酶促因子。它催化 PGH2 转化为 PGD2，PGD2 是平滑肌收缩/松弛所必需的前列腺素，也是血小板聚集的有效抑制剂。除了其血管功能外，PTGDS 还复杂地参与各种中枢神经系统 (CNS) 活动，包括镇静、NREM 睡眠和 PGE2 诱导的异常性疼痛，并可能在少突胶质细胞中发挥抗凋亡作用。该蛋白质表现出广泛的底物结合能力，与胆绿素、胆红素、视黄醛、视黄酸和甲状腺激素等小非底物亲脂性分子相互作用，可能充当有害疏水性分子的清除剂，并充当分泌性视黄醇和甲状腺激素运输者。 PTGDS 参与血液屏障的形成和维持，包括血脑、血视网膜、血-房水和血-睾丸屏障，突出了其在屏障功能中的重要性。此外，它在中枢神经系统和男性生殖系统的成熟和维持中发挥着关键作用。此外，PTGDS 参与 PLA2G3 依赖性肥大细胞成熟，其中未成熟肥大细胞分泌的 PLA2G3 在 PTGDS 上游发挥作用，合成 PGD2。这反过来又通过 PTGDR 促进肥大细胞成熟和脱颗粒，表明它参与免疫反应和细胞成熟。

Rat

HEK293

C-His;C-6*His

P22057/NP_037147.1 (Q25-E189)

25526  [NCBI]

QGHDTVQPNFQQDKFLGRWYSAGLASNSSWFREKKELLFMCQTVVAPSTEGGLNLTSTFLRKNQCETKVMVLQPAGVPGQYTYNSPHWGSFHSLSVVETDYDEYAFLFSKGTKGPGQDFRMATLYSRAQLLKEELKEKFITFSKDQGLTEEDIVFLPQPDKCIQE

Approximately 26-29 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.