1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. BAR/Phosphinothricin N-acetyltransferase 蛋白, Streptomyces hygroscopicus

BAR/Phosphinothricin N-acetyltransferase 蛋白, Streptomyces hygroscopicus

目录号: HY-P70002
COA 产品使用指南

BAR/草铵膦 N-乙酰转移酶蛋白通过促进乙酰辅酶 A 的乙酰基转移,在灭活草铵膦 (PPT) 中发挥关键作用。BAR/Phosphinothricin N-acetyltransferase 蛋白, Streptomyces hygroscopicus 是重组的 BAR/膦丝菌素 N-乙酰转移酶蛋白,由 E. coli 表达,不带标签。BAR/Phosphinothricin N-acetyltransferase 蛋白, Streptomyces hygroscopicus 全长 183 个氨基酸,分子量为 18-20 kDa。

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规格 价格 是否有货 数量
10 μg ¥1070 In-stock
50 μg ¥3000 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The BAR/glufosinate N-acetyltransferase protein plays a key role in inactivating glufosinate (PPT) by promoting acetyl transfer of acetyl-CoA. BAR/Phosphinothricin N-acetyltransferase Protein, Streptomyces hygroscopicus is the recombinant BAR/Phosphinothricin N-acetyltransferase protein, expressed by E. coli , with tag free. The total length of BAR/Phosphinothricin N-acetyltransferase Protein, Streptomyces hygroscopicus is 183 a.a., with molecular weight of 18-20 kDa.

研究背景

BAR/膦丝菌素 N-乙酰转移酶蛋白通过促进乙酰辅酶 A 的乙酰基转移,在灭活膦丝菌素 (PPT) 中发挥关键作用。值得注意的是,它表现出乙酰化去甲基膦丝菌素的能力,同时对 PTT 或谷氨酸保持非活性。这种酶特异性使 BAR 成为赋予草胺膦三肽(PTT 或双丙氨膦)抗性的关键效应子,突出了其在细胞对这些化合物的反应中的功能意义。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Others

表达系统

E. coli

标签

Tag Free

蛋白编号

P16426 (M1-I183)

基因 ID

/

蛋白结构
N-term
BAR (M1-I183)
Accession # P16426
C-term
同用名
rStPhosphinothricin N-acetyltransferase/BAR; Phosphinothricin N-acetyltransferase; PPT N-acetyltransferase; Phosphinothricin-resistance protein; bar
氨基酸序列

MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQEWTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGSTLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGFWQLDFSLPVPPRPVLPVTEI

分子量

18-20 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 12.5 mM Tris-HCl, 50 mM NaCl, ‎‎5% Trehalose, 5% Mannitol, 0.01% Tween 80, 2 mM DTT, 1 mM EDTA, pH 8.5.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

BAR/Phosphinothricin N-acetyltransferase Protein, Streptomyces hygroscopicus 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
BAR/Phosphinothricin N-acetyltransferase Protein, Streptomyces hygroscopicus
目录号:
HY-P70002
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