Plasma kallikrein/KLKB1 蛋白, Mouse (HEK293, His)

Plasma kallikrein/KLKB1 enzymatically cleaves Lys-Arg and Arg-Ser bonds, triggering factor XII activation upon binding to a negatively charged surface.It liberates bradykinin from HMW kininogen and is implicated in the renin-angiotensin system, potentially converting prorenin into renin.This underscores the diverse and essential functions of Plasma kallikrein/KLKB1 in various physiological processes.Plasma kallikrein/KLKB1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Plasma kallikrein/KLKB1 protein, expressed by HEK293 , with C-6*His labeled tag.

血浆激肽释放酶/KLKB1 蛋白通过选择性裂解 Lys-Arg 和 Arg-Ser 键表现出酶活性。与带负电的表面结合后，它会启动因子 XII 的相互激活。此外，该蛋白在从 HMW 激肽原中释放缓激肽方面发挥着至关重要的作用。有趣的是，它与肾素-血管紧张素系统有关，可能有助于肾素原转化为肾素。这种复杂的相互作用强调了血浆激肽释放酶/KLKB1 蛋白在各种生理过程中的多样化和基本功能。

Measured by its ability to cleave 10 μM fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is >300 pmol/min/µg.

Mouse

HEK293

C-6*His

P26262 (G20-A638)

16621

Full Length of Mature Protein

Approximately 80 kDa, due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 20 mM NaAc, 150 mM NaCl, pH 5.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.