PPARD 蛋白, Human (His)

PPARD protein is a ligand-activated transcription factor that critically mediates energy metabolism in adipose tissue. As a receptor, it selectively binds peroxisome proliferators, including hypolipidemic drugs and fatty acids, and preferentially binds polyunsaturated substances. PPARD Protein, Human (His) is the recombinant human-derived PPARD protein, expressed by E. coli, with N-6*His labeled tag.

PPARD 蛋白是一种配体激活的转录因子，是脂肪组织内能量代谢的重要介质。作为受体，它选择性地结合过氧化物酶体增殖剂，包括降血脂药物和脂肪酸，并优先结合多不饱和脂肪酸，例如γ-亚油酸和二十碳五烯酸。配体激活后，受体与靶基因的启动子元件结合，调节脂肪酸的过氧化物酶体β-氧化途径，并充当酰基辅酶A氧化酶基因的转录激活剂。此外，它还可以在配体激活后降低 NPC1L1 的表达。PPARD 与类视黄醇 X 受体形成异二聚体，并通过其 NR LBD 结构域以配体依赖性方式与 CRY1 和 CRY2 相互作用。

Human

E. coli

N-6*His

Q03181 (Q171-Y441)

5467

Partial

QVADLKAFSKHIYNAYLKNFNMTKKKARSILTGKASHTAPFVIHDIETLWQAEKGLVWKQLVNGLPPYKEISVHVFYRCQCTTVETVRELTEFAKSIPSFSSLFLNDQVTLLKYGVHEAIFAMLASIVNKDGLLVANGSGFVTREFLRSLRKPFSDIIEPKFEFAVKFNALELDDSDLALFIAAIILCGDRPGLMNVPRVEAIQDTILRALEFHLQANHPDAQYLFPKLLQKMADLRQLVTEHAQMMQRIKKTETETSLHPLLQEIYKDMY

Approximately 34 kDa, based on SDS-PAGE under reducing conditions.

Greater than 85% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.