PpsA 蛋白, E.coli (His-SUMO)

The PpsA protein plays a crucial role in cellular metabolism by catalyzing the conversion of pyruvate to phosphoenolpyruvate, a key step in glycolysis and gluconeogenesis. This enzyme activity is the basis for the interconversion of metabolic intermediates and contributes to the regulation of intracellular energy production and utilization. PpsA Protein, E.coli (His-SUMO) is the recombinant E. coli-derived PpsA protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of PpsA Protein, E.coli (His-SUMO) is 791 a.a., with molecular weight of ~103.3 kDa.

PpsA 蛋白是一种酶，通过催化丙酮酸磷酸化为磷酸烯醇丙酮酸，在糖酵解和糖异生中发挥关键作用。这种酶活性代表了代谢中间体相互转化的关键步骤，促进丙酮酸进入糖异生途径或其通过糖酵解的进展。PpsA 对丙酮酸的磷酸化是一个可逆反应，有助于调节通过这些代谢途径的总碳通量。这种酶对于维持细胞能量稳态至关重要，并且是根据细胞能量需求微调代谢过程不可或缺的一部分。了解 PpsA 的功能可以为中心碳代谢的动态调节提供有价值的见解。参考资料：所提供的信息基于截至 2022 年 1 月的一般知识，未引用具体参考资料。

E.coli

E. coli

N-His;N-SUMO

P23538 (2S-792K)

946209  [NCBI]

SNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVSVPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQPELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAVKHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQVVFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHGKQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVLVTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENVTVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVGLARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATLGAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFALECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIPSNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRAAKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK

Approximately 103.3 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.