1. 重组蛋白
  2. Biotinylated Proteins
  3. 蛋白 L 蛋白, Finegoldia magna (Biotinylated, HEK293, His-Avi)

蛋白 L 蛋白, Finegoldia magna (Biotinylated, HEK293, His-Avi)

目录号: HY-P78810
COA 产品使用指南

蛋白源自*消化链球菌*,选择性结合免疫球蛋白 kappa 轻链,而不与 Fc 区相互作用。该特性可用于抗体纯化、诊断和研究,以根据轻链组成选择性分离抗体。Protein L 蛋白, Finegoldia magna (Biotinylated, HEK293, His-Avi) 是重组的 蛋白 L 蛋白,由 HEK293 表达,带有 N-His, N-Avi 标签。Protein L 蛋白, Finegoldia magna (Biotinylated, HEK293, His-Avi) 全长 365 个氨基酸,分子量约为 ~50 kDa。

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5 μg ¥550 In-stock
10 μg ¥900 In-stock
50 μg ¥2500 In-stock
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

L protein is derived from *Peptostreptococcus* and selectively binds to the immunoglobulin kappa light chain without interacting with the Fc region. This property can be used in antibody purification, diagnostics, and research to selectively isolate antibodies based on their light chain composition. Protein L Protein, Finegoldia magna (Biotinylated, HEK293, His-Avi) is the recombinant protein L protein, expressed by HEK293 , with N-His, N-Avi labeled tag. The total length of Protein L Protein, Finegoldia magna (Biotinylated, HEK293, His-Avi) is 365 a.a., with molecular weight of ~50 kDa.

研究背景

L 蛋白是一种细菌免疫球蛋白结合蛋白,由*大消化链球菌*产生。它特异性结合免疫球蛋白 (Ig) 的 kappa 轻链,不与 Fc 区相互作用。L 蛋白可应用于抗体纯化、诊断和研究,它能够根据轻链组成选择性分离抗体。这种独特的特性使 Protein L 成为涉及抗体操作和分析的各种生物技术和生物医学应用中的宝贵工具。

生物活性

Measured by its binding ability in a functional ELISA. Immoilized Ipilimumab antibody at 2 μg/mL can bind Biotinylated Protein L. The EC 50 is 1.123-1.761 ng/mL.

种属

Others

表达系统

HEK293

标签

N-His;N-Avi

蛋白编号

D6S9W1 (K106-G470)

基因 ID

/

同用名
RPL; Protein L
分子量

Approximately 50 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Protein L Protein, Finegoldia magna (Biotinylated, HEK293, His-Avi) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Protein L Protein, Finegoldia magna (Biotinylated, HEK293, His-Avi)
目录号:
HY-P78810
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