SBDS 蛋白, Human (His)

The SBDS protein is indispensable for ribosome biogenesis and cooperates with EFL1 to trigger GTP-dependent EIF6 release from 60S preribosomes in the cytoplasm. This activates ribosomes, allowing 80S ribosome assembly and recycling of EIF6 to the nucleus. SBDS Protein, Human (His) is the recombinant human-derived SBDS protein, expressed by E. coli , with N-His labeled tag. The total length of SBDS Protein, Human (His) is 250 a.a., with molecular weight of ~30 kDa.

SBDS 蛋白对于成熟核糖体的形成和核糖体生物合成过程至关重要。它与 EFL1 协同作用，触发细胞质中 60S 前核糖体中 GTP 依赖性的 EIF6 释放。这种释放会激活核糖体，从而组装 80S 核糖体并促进 EIF6 循环回到细胞核。在细胞核中，EIF6 对于 60S rRNA 加工和核输出至关重要。SBDS 蛋白对于正常的蛋白质合成水平也是必需的，并且可能在细胞应激抵抗、DNA 损伤反应和细胞增殖中发挥作用。它与 60S 核糖体亚基结合，并与 NPM1、RPA1、PRKDC、NIP7、EFL1 和 CLN3 相互作用。此外，它还与 60S 核糖体亚基、SBDS 和 EFL1 形成复合物。

Human

E. coli

N-His

Q9Y3A5 (M1-E250)

51119  [NCBI]

MSIFTPTNQIRLTNVAVVRMKRAGKRFEIACYKNKVVGWRSGVEKDLDEVLQTHSVFVNVSKGQVAKKEDLISAFGTDDQTEICKQILTKGEVQVSDKERHTQLEQMFRDIATIVADKCVNPETKRPYTVILIERAMKDIHYSVKTNKSTKQQALEVIKQLKEKMKIERAHMRLRFILPVNEGKKLKEKLKPLIKVIESEDYGQQLEIVCLIDPGCFREIDELIKKETKGKGSLEVLNLKDVEEGDEKFE

Approximately 30 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, 10% Glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.