SDF-1 alpha/CXCL12 蛋白, Human

SDF-1 alpha (CXCL12α) belongs to the CXC chemokine family and is encoded by the CXCL12 gene. SDF-1 alpha mediates cell chemotaxis and tissue repair through CXCR4/CXCR7, activates AMPK to inhibit NLRP3 inflammasome and pyroptosis; SDF-1 alpha promotes autophagy through the PI3K-mTOR pathway, is induced by upstream inflammatory factors such as TNF-α, and recruits integrins downstream to promote cell adhesion^[1]. SDF-1 alpha/CXCL12 Protein, Human is the recombinant human-derived SDF-1 alpha/CXCL12 protein, expressed by E. coli, with tag free.

SDF-1 alpha (stromal cell-derived factor 1 alpha，CXCL12α) 是 CXC 趋化因子家族成员，由 CXCL12 基因编码，含 4 个保守半胱氨酸，形成两个二硫键 (Cys9-Cys34、Cys11-Cys50)，缺乏 ELR (Glu-Leu-Arg) 结构域。SDF-1 alpha 的成熟肽一般含 68 个氨基酸，分子量约 8 kDa，是 SDF-1 的主要活性异构体 (另一异构体为 SDF-1β)^[1]。
SDF-1 alpha 通过受体 CXCR4 介导细胞趋化 (如造血干细胞、T 细胞、单核细胞)，调控组织修复、血管生成及炎症反应。SDF-1 alpha 参与 AMPK 通路：在骨关节炎 (OA) 滑膜成纤维细胞中，SDF-1α 激活 AMPK，抑制 NLRP3 炎症小体 (NLRP3/ASC/caspase-1) 及焦亡 (GSDMD/IL-1β)。SDF-1 alpha 也参与 PI3K-mTOR 通路：通过 CXCR4 激活 PI3K-mTOR，促进自噬 (如软骨细胞中 LC3II 升高、p62 降低)，但该通路不直接介导炎症抑制。SDF-1 alpha 先受到上游受炎症因子 (如 TNF-α、IL-1β) 诱导，通过下游的 CXCR4 招募整合素 (如 VLA-4) 促进细胞黏附与迁移^[1]。
SDF-1 alpha 可用于骨关节炎 (OA)、炎症、自噬等疾病或机制的研究。例如，在关节内注射 SDF-1 alpha 可缓解胶原酶诱导的小鼠 OA，抑制滑膜炎症及软骨降解；SDF-1 alpha 在软骨细胞中通过 CXCR4/mTOR 轴促进自噬，保护细胞免受应激损伤^[1]。SDF-1 alpha 在干细胞生物学中，还调控造血干细胞归巢 (如骨髓移植中增强 CXCR4 表达)^[2]。

SDF-1 alpha 相关
SDF-1 alpha (人源；20 ng/mL；24 h) 可促进骨关节炎 (OA) 滑膜成纤维细胞 (FLS) 增殖，而 50 ng/mL 和 100 ng/mL 对细胞增殖无显著影响^[1]。
SDF-1 alpha (人源；20、50、100 ng/mL；24 h) 能显著下调 OA FLS 中 NLRP3 炎症小体 (NLRP3、ASC、caspase-1) 及焦亡标志物 (GSDMD、IL-1β) 的基因和蛋白表达，其中 100 ng/mL 效果最显著^[1]。
SDF-1 alpha (人源；100 ng/mL；1-24 h) 激活 AMPK 信号通路，p-AMPK 蛋白表达在 3 h 达峰值，6 h 后逐渐下降^[1]。
SDF-1 alpha (人源；100 ng/mL；24 h) 对 AMPK 信号通路的激活作用，可被 AMPK 抑制剂 10 μmol/mL Dorsomorphin (HY-13418A) 逆转，而解除 NL

SDF-1 alpha (人源；120 ng/kg；关节内注射；每周 2 次；7 周) 在胶原酶诱导的骨关节炎 (OA) 小鼠模型中，显著减少关节间隙狭窄和骨赘形成，下调滑膜组织中 NLRP3 炎症小体 (NLRP3、ASC、caspase-1) 及焦亡标志物 (GSDMD、IL-1β) 的表达^[1]。

1. Full biological activity determined by a chemotaxis bioassay using PHA and rHuIL-2 activated human peripheral blood T-lymphocytes is in a concentration range of 20-80 ng/mL.
2. Measured by its ability to chemoattract IL-2-activated human T cells. The ED₅₀ for this effect is approximately ≤45.17 ng/mL, corresponding to a specific activity is ≥2.214×10⁴ U/mg.

Human

E. coli

Tag Free

P48061-2 (K22-K89)

6387  [NCBI]

Full Length of Isoform-2 Mature Protein

KPVSLSYRCPCRFFESHVARANVKHLKILNTPNCALQIVARLKNNNRQVCIDPKLKWIQEYLEKALNK

Approximately 8-10.0 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4 or 20 mM PB, 130 mM NaCl, pH 7.3.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Wang S, et al. Exogenous stromal cell-derived factor-1 (SDF-1) suppresses the NLRP3 inflammasome and inhibits pyroptosis in synoviocytes from osteoarthritic joints via activation of the AMPK signaling pathway. Inflammopharmacology. 2021 Jun;29(3):695-704.  [Content Brief]

  [2]. Hayakawa J, et al. Dextran sulfate and stromal cell derived factor-1 promote CXCR4 expression and improve bone marrow homing efficiency of infused hematopoietic stem cells. J Nippon Med Sch. 2009 Aug;76(4):198-208.  [Content Brief]

  [3]. Warner JA, et al. Human cytomegalovirus infection inhibits CXCL12- mediated migration and invasion of human extravillous cytotrophoblasts. Virol J. 2012 Nov 1;9:255.  [Content Brief]

  [4]. Yadav SS, et al. CXCL12 is a key regulator in tumor microenvironment of cervical cancer: an in vitro study. Clin Exp Metastasis. 2016 Jun;33(5):431-9.  [Content Brief]

  [5]. Hoh BL, et al. Stromal cell-derived factor-1 promoted angiogenesis and inflammatory cell infiltration in aneurysm walls. J Neurosurg. 2014 Jan;120(1):73-86.  [Content Brief]