Serpin G1 蛋白, Mouse (HEK293, His)

Serpin G1 protein, also known as C1 inhibitor, regulates complement activation, coagulation, fibrinolysis, and kinin production. It forms an inactive complex with C1r or C1s protease and controls C1 complex activation. Serpin G1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Serpin G1 protein, expressed by HEK293 , with C-6*His labeled tag.

Serpin G1 蛋白，也称为 C1 抑制剂，在调节补体激活、凝血、纤维蛋白溶解和激肽生成等重要生理途径中发挥着重要作用。它与 C1r 或 C1s 蛋白酶形成蛋白水解无活性复合物，从而控制 C1 复合物的激活。Serpin G1 蛋白可有效抑制 FXIIa，还可能抑制胰凝乳蛋白酶和激肽释放酶。此外，它还与 MASP1 交互。通过发挥其抑制作用，丝氨酸蛋白酶抑制剂 G1 蛋白有助于维持这些复杂途径的平衡，确保正常的生理功能并防止可能导致病理状况的过度激活。

Measured by its ability to inhibit Recombinant Human Complement Component C1s (HY-P700696) cleavage of a colorimetric peptide substrate, N-carbobenzyloxy-Lys-ThioBenzyl ester (Z-K-SBzl). The IC₅₀ value is 10.49 nM.

Mouse

HEK293

C-6*His

P97290 (A20-G504)

12258  [NCBI]

Partial

AFSDPEATSHSTQDPLEAQAKSRESFPERDDSWSPPEPTVLPSTWPTTSVAITITNDTMGKVANESFSQHSQPAAQLPTDSPGQPPLNSSSQPSTASDLPTQATTEPFCPEPLAQCSDSDRDSSEAKLSEALTDFSVKLYHAFSATKMAKTNMAFSPFSIASLLTQVLLGAGDSTKSNLESILSYPKDFACVHQALKGFSSKGVTSVSQIFHSPDLAIRDTYVNASQSLYGSSPRVLGPDSAANLELINTWVAENTNHKIRKLLDSLPSDTCLVLLNAVYLSAKWKITFEPKKMMAPFFYKNSMIKVPMMSSVKYPVAQFDDHTLKAKVGQLQLSHNLSFVIVVPVFPKHQLKDVEKALNPTVFKAIMKKLELSKFLPTYLTMPHIKVKSSQDMLSVMEKLEFFDFTYDLNLCGLTEDPDLQVSAMKHETVLELTESGVEAAAASAISFGRSLPIFEVQRPFLFLLWDQQHRFPVFMGRVYDPRG

92-95 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.