TFEB 蛋白, Human (N-His)

TFEB protein is a transcription factor that subtly regulates lysosomal function, autophagy, lipid catabolism, energy metabolism, and immune responses. It recognizes E-box and CLEAR-box sequences and controls gene expression based on nutrient availability. TFEB protein, Human (N-His) is the recombinant human-derived TFEB protein, expressed by E. coli , with N-10*His labeled tag.

TFEB 蛋白作为关键转录因子协调多种细胞过程，包括溶酶体生物发生、自噬、溶酶体胞吐作用、脂质分解代谢、能量代谢和免疫反应。识别 E-box 序列后，TFEB 的有效 DNA 结合需要与其自身或其他 MiT/TFE 家族成员（如 TFE3 或 MITF）进行二聚化。它通过 MTOR 介导的磷酸化对氨基酸可用性做出反应，促进细胞质保留和失活。相反，饥饿或溶酶体应激期间的 MTOR 抑制会诱导 TFEB 去磷酸化，从而导致核定位和转录激活。TFEB 特异性结合 CLEAR-box 序列，激活溶酶体基因的表达并调节溶酶体定位。此外，它还能积极调节自噬，促进 T 细胞依赖性抗体反应，并在胎盘血管化中发挥作用。为了应对细菌感染，TFEB 介导衣康酸诱导的烷基化，激活溶酶体生物发生并帮助细菌清除。值得注意的是，艾曲波帕药物通过与 bHLH 结构域结合并破坏 DNA 结合来抑制 TFEB。

Human

E. coli

N-10*His

P19484-1(M1-L476)

7942

Full Length of Isoform-1

MASRIGLRMQLMREQAQQEEQRERMQQQAVMHYMQQQQQQQQQQLGGPPTPAINTPVHFQSPPPVPGEVLKVQSYLENPTSYHLQQSQHQKVREYLSETYGNKFAAHISPAQGSPKPPPAASPGVRAGHVLSSSAGNSAPNSPMAMLHIGSNPERELDDVIDNIMRLDDVLGYINPEMQMPNTLPLSSSHLNVYSSDPQVTASLVGVTSSSCPADLTQKRELTDAESRALAKERQKKDNHNLIERRRRFNINDRIKELGMLIPKANDLDVRWNKGTILKASVDYIRRMQKDLQKSRELENHSRRLEMTNKQLWLRIQELEMQARVHGLPTTSPSGMNMAELAQQVVKQELPSEEGPGEALMLGAEVPDPEPLPALPPQAPLPLPTQPPSPFHHLDFSHSLSFGGREDEGPPGYPEPLAPGHGSPFPSLSKKDLDLMLLDDSLLPLASDPLLSTMSPEASKASSRRSSFSMEEGDVL

Approximately 57.0 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from sterile 20 mM PB, 150 mM Nacl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.