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  3. TFPI 蛋白, Rabbit (HEK293, His)

TFPI 蛋白, Rabbit (HEK293, His)

目录号: HY-P700431
产品使用指南

TFPI 蛋白直接抑制因子 X (Xa),并以 Xa 依赖性方式阻碍 VIIa/组织因子的活性,可能是通过与 Xa、TFPI、VIIa 和组织因子形成四元复合物。这种多功能蛋白具有抗血栓特性,并与血浆中的脂蛋白结合,突出了其在调节凝血途径中的关键作用。TFPI 蛋白, Rabbit (HEK293, His) 是重组的 TFPI 蛋白,由 HEK293 表达,带有 N-10*His 标签。TFPI 蛋白, Rabbit (HEK293, His) 全长 276 个氨基酸,分子量为 35.4 kDa。

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描述

TFPI Protein inhibits factor X (Xa) directly and, in an Xa-dependent manner, hinders the activity of VIIa/tissue factor, possibly by forming a quaternary complex with Xa, TFPI, VIIa, and tissue factor. This multifunctional protein has antithrombotic properties and associates with lipoproteins in plasma, highlighting its crucial role in regulating the coagulation pathway. TFPI Protein, Rabbit (HEK293, His) is the recombinant Rabbit-derived TFPI protein, expressed by HEK293 , with N-10*His labeled tag. The total length of TFPI Protein, Rabbit (HEK293, His) is 276 a.a., with molecular weight of 35.4 kDa.

研究背景

TFPI(组织因子途径抑制剂)对因子 X (Xa) 产生直接抑制作用,并以 Xa 依赖性方式阻碍 VIIa/组织因子的活性,可能是通过形成涉及 Xa、TFPI、VIIa 和组织因子。这种多功能蛋白质表现出抗血栓特性,并表现出与血浆中脂蛋白结合的亲和力,突出了其在调节凝血途径复杂动态方面的关键作用。

种属

Rabbit

表达系统

HEK293

标签

N-10*His

蛋白编号

P19761 (A25-T300)

基因 ID

100009401  [NCBI]

蛋白结构
N-term
10*His
TFPI (A25-T300)
Accession # P19761
C-term
同用名
TFPI; tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor); LACI; tissue factor pathway inhibitor; EPI; extrinsic pathway inhibitor; TFI; TFPI1; anti-convertin;
氨基酸序列

AAEEDEEFTNITDIKPPLQKPTHSFCAMKVDDGPCRAYIKRFFFNILTHQCEEFIYGGCEGNENRFESLEECKEKCARDYPKMTTKLTFQKGKPDFCFLEEDPGICRGYITRYFYNNQSKQCERFKYGGCLGNLNNFESLEECKNTCENPTSDFQVDDHRTQLNTVNNTLINQPTKAPRRWAFHGPSWCLPPADRGLCQANEIRFFYNAIIGKCRPFKYSGCGGNENNFTSKKACITACKKGFIPKSIKGGLIKTKRKKKKQPVKITYVETFVKKT

分子量

35.4 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

TFPI Protein, Rabbit (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TFPI Protein, Rabbit (HEK293, His)
目录号:
HY-P700431
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