Tissue Factor 蛋白, Rabbit (HEK293, His)

Tissue Factor Protein initiates blood coagulation by binding to coagulation factors. It activates clotting cascades and interacts with cells to promote thrombosis and inflammation. Understanding Tissue Factor Protein's functions is crucial for developing treatments for cardiovascular diseases and blood disorders. Tissue Factor Protein, Rabbit (HEK293, His) is the recombinant Rabbit-derived Tissue Factor protein, expressed by HEK293 , with C-His labeled tag. The total length of Tissue Factor Protein, Rabbit (HEK293, His) is 218 a.a., with molecular weight of ~26.4 kDa.

组织因子 (TF) 作为血液凝固的引发剂，与循环因子 VII 或 VIIa 形成复合物。这种 [TF:VIIa] 复合物通过特定的有限蛋白水解作用，激活因子 IX 或 X。在正常止血中，TF 通过促进细胞表面凝血蛋白酶级联的组装和传播而发挥关键作用。此外，TF 与 HSPE 相互作用，这种相互作用在肝素的阻碍下，促进了活化因子 X 的生成，最终有助于在活化因子 VII 存在的情况下激活凝血。这强调了 TF 在凝血调节过程中的复杂参与。

Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate Boc-VPR-AMC. The AC₅₀ is 5.368 μg/mL, as measured under the described conditions.

Rabbit

HEK293

C-His

P24055/NP_001075760.1 (A33-M250)

100009127  [NCBI]

ADTTGRAYNLTWKSTNFKTILEWEPKSIDHVYTVQISTRLENWKSKCFLTAETECDLTDEVVKDVGQTYMARVLSYPARNGNTTGFPEEPPFRNSPEFTPYLDTNLGQPTIQSFEQVGTKLNVTVQDARTLVRRNGTFLSLRAVFGKDLNYTLYYWRASSTGKKTATTNTNEFLIDVDKGENYCFSVQAVIPSRKRKQRSPESLTECTSREQGRAREM

Approximately 35-55 kDa due to the glycosylation

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.