1. 重组蛋白
  2. Receptor Proteins Enzymes & Regulators
  3. Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. TAM Receptor
  5. TYRO3
  6. TYRO3/DTK 蛋白, Mouse (HEK293, His)

TYRO3/DTK 蛋白, Mouse (HEK293, His)

目录号: HY-P77863
产品使用指南

TYRO3/DTK 蛋白是一种受体酪氨酸激酶,通过 TULP1 或 GAS6 等配体转导信号来调节细胞存活、迁移和分化。配体结合诱导 TYRO3 二聚化和自磷酸化,为下游分子创建对接位点。TYRO3/DTK 蛋白, Mouse (HEK293, His) 是重组的 TYRO3/DTK 蛋白,由 HEK293 表达,带有 C-His 标签。TYRO3/DTK 蛋白, Mouse (HEK293, His) 全长 389 个氨基酸,分子量为 60-75 kDa。

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描述

TYRO3/DTK protein is a receptor tyrosine kinase that transduces signals through ligands such as TULP1 or GAS6 to regulate cell survival, migration and differentiation. Ligand binding induces TYRO3 dimerization and autophosphorylation, creating docking sites for downstream molecules. TYRO3/DTK Protein, Mouse (HEK293, His) is the recombinant mouse-derived TYRO3/DTK protein, expressed by HEK293 , with C-His labeled tag. The total length of TYRO3/DTK Protein, Mouse (HEK293, His) is 389 a.a., with molecular weight of 60-75 kDa.

研究背景

TYRO3/DTK 蛋白是一种受体酪氨酸激酶,通过与各种配体(包括 TULP1 或 GAS6)结合来转导来自细胞外基质的信号。它在调节许多生理过程中发挥着至关重要的作用,包括细胞存活、迁移和分化。配体在细胞表面结合后,TYRO3 在其胞内结构域上发生二聚化和自磷酸化,为下游信号分子创建对接位点。激活后,TYRO3 与 PIK3R1 相互作用,增强 PI3 激酶活性并激活 AKT 生存途径,导致 NF-kappa-B 核转位和 NF-kappa-B 调节基因的转录上调。TYRO3 信号传导涉及神经元免受兴奋性毒性损伤、血小板聚集和细胞骨架重组等过程。此外,TYRO3 在通过激活 STAT1 抑制 Toll 样受体 (TLR) 介导的先天免疫反应中发挥着至关重要的作用,STAT1 选择性诱导细胞因子信号转导 SOCS1 和 SOCS3 抑制剂的产生。

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

P55144-2 (R31-D419)

基因 ID

22174  [NCBI]

蛋白结构
N-term
TYRO3 (R31-D419)
Accession # P55144-2
His
C-term
同用名
BYK; DTK; RSE; SKY; TIF; TYRO3; Brt; Rse
分子量

60-75 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TYRO3/DTK Protein, Mouse (HEK293, His)
目录号:
HY-P77863
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