UBB 蛋白, Human

UBB proteins are central regulators of the formation of covalently linked isopeptide bonds in different states: monoubiquitin, polyubiquitin chains, or linear polyubiquitin. UBB Protein, Human is the recombinant human-derived UBB protein, expressed by E. coli , with tag free. The total length of UBB Protein, Human is 76 a.a., with molecular weight of ~7.0 kDa.

UBB 蛋白是细胞调节中的关键参与者，它要么与其他蛋白共价连接，要么以游离、非锚定状态存在。在共价结合形式中，UBB 通过异肽键与靶蛋白结合，表现为单泛素、通过不同 Lys 残基连接的多泛素链或在 Met 残基起始的线性多泛素链。多聚泛素链的功能多种多样，并且取决于所涉及的特定 Lys 残基：Lys-6 连接的泛素可能有助于 DNA 修复，Lys-11 连接涉及内质网相关降解 (ERAD) 和细胞周期调节，Lys-29 连接与蛋白毒性应激反应和细胞周期相关，Lys-33 连接参与激酶修饰，Lys-48 连接对于蛋白酶体的蛋白质降解至关重要，Lys-63 连接在内吞作用中发挥作用、DNA 损伤反应和 NF-kappa-B 激活。由 Met 起始的线性多泛素链与细胞信号传导相关。虽然 UBB 通常与 Lys 残基缀合，但极少数情况涉及与 Cys 或 Ser 残基缀合。在非锚定状态下，游离多聚泛素发挥不同的作用，例如激活蛋白激酶和参与信号传导过程。此外，UBB 与 SKP1-KMD2A 和 SKP1-KMD2B 复合物相互作用，强调其参与特定的细胞途径和蛋白质相互作用。

Human

E. coli

Tag Free

P0CG47 (M153-G228)

7314  [NCBI]

MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG

Approximately 7.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.