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  3. Fluo-4 AM

Fluo-4 AM 是一种常用的检测细胞内 Ca2+ 浓度的探针。

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Fluo-4 AM Chemical Structure

Fluo-4 AM Chemical Structure

CAS No. : 273221-67-3

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规格 价格 是否有货 数量
100 μg ¥1500
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Top Publications Citing Use of Products

    Fluo-4 AM purchased from MCE. Usage Cited in: Front Pharmacol. 2022 May 17;13:855626.  [Abstract]

    Intracellular Ca2+ is measured by Fluo-4/AM (for 30 min) with flow cytometry.

    Fluo-4 AM purchased from MCE. Usage Cited in: Cell Death Discov. 2021 Feb 10;7(1):31.  [Abstract]

    The cardiomyocytes are isolated by enzyme digestion and loaded with Fluo 4-AM (5 μM) at 37 °C for 20 min. Fluorescence changes of Fluo 4-AM are recorded by confocal microscopy at 25 °C.

    Fluo-4 AM purchased from MCE. Usage Cited in: Oxid Med Cell Longev. 2021 Feb 10;2021:8884922.

    NP cells are incubated with 5 μM Fura-4-AM for 30 min at 37°C in the dark. Intracellular Ca2+ levels measured using the specific Ca2+-sensitive fluorescent indicator Fura-4-AM and analyzed via fluorescence microscopy.
    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Fluo-4 AM is a cell-permeable Ca2+ indicator[1].

    体外研究
    (In Vitro)

    Fluo-4 AM 是一种荧光染料 (λex=494 nm,λem=516 nm)。预装 Fuo-4 AM,可以观察到非常明亮的荧光图像。在使用 fluo-3 AM 加载细胞进行的平行实验中,得到的荧光图像虽然在这种情况下清晰可辨,但不太亮[1]
    指南 (以下是我们推荐的方案。本方案仅提供指南,应根据您的具体需要进行修改)。
    1. 计数细胞,从每个样本 (对照和实验) 中取106个细胞。
    2. 收集细胞 (5分钟,3000×g,4℃),用PBS洗涤一次。
    3. 用 0.5-ml PBS重悬细胞,加入 0.5 ul Fluo-4- AM (1 mM 库存) 至最终浓度为 1 μM。37℃ 孵育 1 小时。
    4. 用 PBS 洗涤细胞 3 次 (2分钟,3000×g),最后用 1 ml PBS重悬。分成 2 个流式细胞仪管,每管 0.5 ml。
    5. 用流式细胞术评估染色,并用 CellQuest 软件等软件分析数据。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    分子量

    1096.94

    Formula

    C51H50F2N2O23

    CAS 号
    性状

    固体

    颜色

    Brown to reddish brown

    Emission (Em)

    526

    Excitation (Ex)

    485

    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式

    -20°C, sealed storage, away from moisture and light

    *该产品在溶液状态不稳定,建议您现用现配,即刻使用。

    纯度 & 产品资料

    纯度: ≥99.0%

    染色示例
    参考文献
    Cell Assay
    [1]

    For measuring fluorescence from cells in suspension, dilutions to 2 to 3×106 cells are made, from cultures of rat basophilic leukemia (RBL) cells. Cells are incubated in suspension in 1 μM dye (including Fluo-4 AM) for 30 min at 37°C. Cell suspensions are then transferred to cuvets for measurements of fluorescence emission intensity by spectrofluorometer[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    参考文献

    Fluo-4 AM 相关分类

    • 摩尔计算器

    • 稀释计算器

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量   浓度   体积   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    产品名称:
    Fluo-4 AM
    目录号:
    HY-101896
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