Pyranine (Synonyms: 溶剂绿7; HPTS; Solvent Green 7)

目录号: HY-D0023 纯度: ≥98.0%: Data Sheet SDS COA 产品使用指南
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Pyranine (HPTS; Solvent Green 7) 是一种 pH 敏感的荧光指示剂。Pyranine 是一类用于 Cu⁺ 离子的荧光化学传感器 (λex=450 nm，λem=510 nm)。

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Pyranine Chemical Structure

CAS No. : 6358-69-6

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规格	价格	是否有货
10 mM * 1 mL in DMSO	￥550	In-stock
500 mg	￥500	In-stock
1 g	￥800	In-stock
5 g		询价
10 g		询价

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Other Forms of Pyranine:

Pyranine hydrate 询价
8-Hydroxypyrene-1,3,6-trisulfonic acid 询价

MCE 顾客使用本产品发表的 2 篇科研文献

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生物活性
实验参考方法
纯度 & 产品资料
参考文献

生物活性

Pyranine (HPTS; Solvent Green 7) is a pH-sensitive fluorescent indicator. Pyranine acts as a class of fluorescent chemosensor for the Cu⁺ ion(λex=450 nm, λem=510 nm)^[1]^[2]^[3].

体外研究
(In Vitro)

即使在竞争环境中，Pyranine 也能区分来自 Cu⁺ 离子的阳离子范围。Pyranine 对 Cu⁺ 离子表现出快速的荧光响应(t_1/2=1.66 分钟)，微摩尔检测限使其能够检测环境样品中的离子。观察到的 Pyranine 与 Cu⁺ 之间的络合化学计量为 2:1^[1]。
研究了 pH 敏感的荧光指示剂 Pyranine，以确定其作为活体角膜和前房探针的有效性。局部给药后，在角膜和前房中达到足够的浓度；可以通过荧光光度法进行数小时的测量。pH 值是通过测量两个激发波长 I463/I404 下的荧光强度比值来计算的，该测量值取决于 pH 值，但与荧光团浓度及其他可能改变强度的变量无关。在兔眼中，我们观察到角膜和前房中的吡喃氨酸会随着眼睑闭合和佩戴隐形眼镜而发生易于测量的荧光比值变化，这表明其对 pH 值的轻微变化较为敏感^[2]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

分子量

524.39

Formula

C₁₆H₇Na₃O₁₀S₃

CAS 号

6358-69-6

性状

固体

颜色

Light yellow to yellow

Emission (Em)

510

Excitation (Ex)

450

中文名称

溶剂绿7

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

溶解性数据

细胞实验：

H₂O 中的溶解度 : 20 mg/mL (38.14 mM; 超声助溶 (<60°C))

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.9070 mL	9.5349 mL	19.0698 mL
5 mM	0.3814 mL	1.9070 mL	3.8140 mL
10 mM	0.1907 mL	0.9535 mL	1.9070 mL

查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

摩尔计算器
稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

浓度 _(start)

体积 _(start)

浓度 _(final)

体积 _(final)

动物实验：

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

方案一

请依序添加每种溶剂： PBS
Solubility: 25 mg/mL (47.67 mM); 澄清溶液; 超声加热助溶

动物溶解方案计算器

请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

每只动物的给药体积

μL

动物数量

只

由于实验过程有损耗，建议您多配一只动物的量

计算结果

工作液所需浓度 : mg/mL

该产品水溶性佳，请具体参考实测水 / PBS / Saline 中的溶解度数据。

您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。

纯度 & 产品资料

纯度: ≥98.0%

选择批次：

Data Sheet (647 KB) SDS (393 KB)

COA (298 KB) HNMR (257 KB)

产品使用指南 (1538 KB)

参考文献

[1]. Saha T, et al. In vitro sensing of Cu(+) through a green fluorescence rise of pyranine. Photochem Photobiol Sci. 2014 Oct;13(10):1427-33. [Content Brief]

[2]. Thomas JV, et al. The fluorescent indicator pyranine is suitable for measuring stromal and cameral pH in vivo. Exp Eye Res. 1990 Mar;50(3):241-9. [Content Brief]

[3]. Evingür GA, et al. Kinetic models for the dynamical behavior of polyacrylamide (PAAm)-κ-carrageenan (κC) composite gels. J Biol Phys. 2015 Jan;41(1):37-47. [Content Brief]

Cell Assay ^[3]	PAAm-κC composite gels are prepared by free-radical copolymerization using 0.71 g of AAm, 0.01 g of BIS (N, N’-methylenebisacrylamide), 0.008 g of APS (ammonium persulfate) and 2 µl of TEMED (tetramethylethylenediamine) are dissolved in 5 mL of distilled water (pH 6.5) by heating. The heated mixture solution is held at 80°C. Then different amounts of κ-carrageenan (0.5, 1, 1.5, 2, 2.5 and 3 (w/v) % κC) are added. Pyranine (Py) is used in the PAAm–κC composites as a fluorescence probe, which is a derivative of pyrene possessing three SO₃^- groups, which can form bonds with positive charges on the gel. Pyranine (Py) concentration is kept constant at 4×10^-4 M, for all experiments. The solution is stirred (200 rpm) for 15 min to achieve a homogenous sample solution. All samples are deoxygenated by bubbling nitrogen for 10 min just before the polymerization process. The drying and swelling experiments of disc-shaped PAAm–κC composite gels prepared are performed in air and in water, respectively, at various temperatures (30, 40, 50, and 60°C). A model LS-50 spectrometer equipped with a temperature controller is used for fluorescence intensity measurements, which are made at a 90° position and spectral bandwidths are kept at 5 nm. Disc-shaped gel samples are placed on the wall of a 1-cm path-length square quartz cell filled with air and/or water for the drying and swelling experiments^[3]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献	[1]. Saha T, et al. In vitro sensing of Cu(+) through a green fluorescence rise of pyranine. Photochem Photobiol Sci. 2014 Oct;13(10):1427-33. [Content Brief] [2]. Thomas JV, et al. The fluorescent indicator pyranine is suitable for measuring stromal and cameral pH in vivo. Exp Eye Res. 1990 Mar;50(3):241-9. [Content Brief] [3]. Evingür GA, et al. Kinetic models for the dynamical behavior of polyacrylamide (PAAm)-κ-carrageenan (κC) composite gels. J Biol Phys. 2015 Jan;41(1):37-47. [Content Brief]

Cell Assay
^[3]

PAAm-κC composite gels are prepared by free-radical copolymerization using 0.71 g of AAm, 0.01 g of BIS (N, N’-methylenebisacrylamide), 0.008 g of APS (ammonium persulfate) and 2 µl of TEMED (tetramethylethylenediamine) are dissolved in 5 mL of distilled water (pH 6.5) by heating. The heated mixture solution is held at 80°C. Then different amounts of κ-carrageenan (0.5, 1, 1.5, 2, 2.5 and 3 (w/v) % κC) are added. Pyranine (Py) is used in the PAAm–κC composites as a fluorescence probe, which is a derivative of pyrene possessing three SO₃^- groups, which can form bonds with positive charges on the gel. Pyranine (Py) concentration is kept constant at 4×10^-4 M, for all experiments. The solution is stirred (200 rpm) for 15 min to achieve a homogenous sample solution. All samples are deoxygenated by bubbling nitrogen for 10 min just before the polymerization process. The drying and swelling experiments of disc-shaped PAAm–κC composite gels prepared are performed in air and in water, respectively, at various temperatures (30, 40, 50, and 60°C). A model LS-50 spectrometer equipped with a temperature controller is used for fluorescence intensity measurements, which are made at a 90° position and spectral bandwidths are kept at 5 nm. Disc-shaped gel samples are placed on the wall of a 1-cm path-length square quartz cell filled with air and/or water for the drying and swelling experiments^[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

参考文献

[1]. Saha T, et al. In vitro sensing of Cu(+) through a green fluorescence rise of pyranine. Photochem Photobiol Sci. 2014 Oct;13(10):1427-33. [Content Brief]

[2]. Thomas JV, et al. The fluorescent indicator pyranine is suitable for measuring stromal and cameral pH in vivo. Exp Eye Res. 1990 Mar;50(3):241-9. [Content Brief]

[3]. Evingür GA, et al. Kinetic models for the dynamical behavior of polyacrylamide (PAAm)-κ-carrageenan (κC) composite gels. J Biol Phys. 2015 Jan;41(1):37-47. [Content Brief]

Pyranine 相关分类

Others

完整储备液配制表

可选溶剂	浓度溶剂体积质量	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	1.9070 mL	9.5349 mL	19.0698 mL	47.6744 mL
	5 mM	0.3814 mL	1.9070 mL	3.8140 mL	9.5349 mL
	10 mM	0.1907 mL	0.9535 mL	1.9070 mL	4.7674 mL
	15 mM	0.1271 mL	0.6357 mL	1.2713 mL	3.1783 mL
	20 mM	0.0953 mL	0.4767 mL	0.9535 mL	2.3837 mL
	25 mM	0.0763 mL	0.3814 mL	0.7628 mL	1.9070 mL
	30 mM	0.0636 mL	0.3178 mL	0.6357 mL	1.5891 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

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Keywords:

Pyranine6358-69-6HPTS Solvent Green 7溶剂绿7Solvent Green7Solvent Green 7Solvent Green-7Fluorescent DyepHfluorescentindicatorCu+Inhibitorinhibitorinhibit

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Pyranine (Synonyms: 溶剂绿7; HPTS; Solvent Green 7)

Customer Review

Other Forms of Pyranine:

MCE 顾客使用本产品发表的 2 篇科研文献

Pyranine 相关产品

•同靶点产品:

•同靶点蛋白产品:

生物活性

实验参考方法

纯度 & 产品资料

参考文献

摩尔计算器

稀释计算器

Pyranine 相关分类

完整储备液配制表

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Pyranine (Synonyms: 溶剂绿7; HPTS; Solvent Green 7)

Customer Review

Other Forms of Pyranine:

Pyranine 相关抗体

MCE 顾客使用本产品发表的 2 篇科研文献

Pyranine 相关产品

•同靶点产品:

•同靶点蛋白产品:

生物活性

实验参考方法

纯度 & 产品资料

参考文献

Pyranine 相关抗体:

摩尔计算器

稀释计算器

Pyranine 相关分类

完整储备液配制表

Keywords:

您最近查看的产品:

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