2. Others
  3. Fluorescent Dye
  4. RT-AM

RT-AM是real thiol前体活性分子。Real Thiol 是一种基于可逆反应的荧光探针，可以定量监测活细胞中谷胱甘肽的实时动态变化。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

RT-AM Chemical Structure

RT-AM Chemical Structure

CAS No. : 2280796-94-1

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2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 价格 是否有货 数量
1 mg ￥3500
In-stock
5 mg   询价  
10 mg   询价  

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Customer Review

Other Forms of RT-AM:

RT-AM 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

RT-AM is a pro-agent real thiol. Real Thiol is a reversible reaction-based fluorescent probe which can quantitatively monitor the real-time glutathione dynamics in living cells.

体外研究
(In Vitro)

To enhance the cell permeability of Real Thiol, the carboxylic acid groups is converted to acetoxymethyl (AM) esters, which are readily hydrolysed by esterases to regenerate Real Thiol inside cells. For cells treated with the RT-AM, real-time ratiometric images of the rapid changes of intracellular GSH concentrations in single cells can be generated by dividing the fluorescence intensity values for the 405 nm channel by the 488 nm channel at each corresponding pixel[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

RT-AM 相关抗体:
分子量

555.49

Formula

C26H25N3O11
CAS 号
性状

固体

颜色

Pink to red

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 2 years
-20°C 1 year
溶解性数据
In Vitro: 

DMSO 中的溶解度 : 25 mg/mL (45.01 mM; 超声助溶; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 1.8002 mL 9.0011 mL 18.0021 mL
5 mM 0.3600 mL 1.8002 mL 3.6004 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: 98.07%

COA (196 KB) HNMR (270 KB) LCMS (101 KB)

产品使用指南 (1538 KB)
参考文献
Cell Assay
[1]

The HeLa cell line is grown in DMEM media supplemented with 10% FBS and 1% 1003 Pen Strep. Cells are cultured under a controlled atmosphere (37°C, 5% CO2). Glass bottom dishes are used for cell culture due to confocal scanning requirements. Cells are treated with RT-AM (1 μM with 1% DMSO in DMEM) for 10-15 min before imaging. Confocal images are acquired with 405 nm laser/418–495 nm filter and 488 nm laser/499–615 nm filter. All the microscope settings are kept consistent in each[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

RT-AM 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 2 years; -20°C, 1 year。-80°C储存时，请在2年内使用， -20°C储存时，请在1年内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8002 mL 9.0011 mL 18.0021 mL 45.0053 mL
5 mM 0.3600 mL 1.8002 mL 3.6004 mL 9.0011 mL
10 mM 0.1800 mL 0.9001 mL 1.8002 mL 4.5005 mL
15 mM 0.1200 mL 0.6001 mL 1.2001 mL 3.0004 mL
20 mM 0.0900 mL 0.4501 mL 0.9001 mL 2.2503 mL
25 mM 0.0720 mL 0.3600 mL 0.7201 mL 1.8002 mL
30 mM 0.0600 mL 0.3000 mL 0.6001 mL 1.5002 mL
40 mM 0.0450 mL 0.2250 mL 0.4501 mL 1.1251 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RT-AM2280796-94-1Fluorescent DyeInhibitorinhibitorinhibit

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产品名称：
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目录号：
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