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  4. Real Thiol

Real Thiol 是一种基于可逆反应的荧光探针，可以定量监测活细胞中谷胱甘肽的实时动态变化。

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Real Thiol Chemical Structure

Real Thiol Chemical Structure

CAS No. : 2280796-90-7

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规格 价格 是否有货 数量
1 mg ￥3500
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Customer Review

Other Forms of Real Thiol:

Real Thiol 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 实验参考方法

  • 纯度 & 产品资料

  • 参考文献

生物活性

Real Thiol is a reversible reaction-based fluorescent probe which can quantitatively monitor the real-time glutathione dynamics in living cells.

体外研究
(In Vitro)

Real Thiol (RT) shows ratiometric fluorescence responses with a wide dynamic range when reacting with Glutathione (GSH). Real Thiol and its GSH adduct (RT-GSH) shows fluorescence maxima at 487 and 562?nm with excitation wavelengths at 405 and 488?nm, respectively. The Kd for the reaction between Real Thiol and GSH is 3.7?mM. Real Thiol has much improved quantum yields and photostability. The wide dynamic range of Real Thiol allows for monitoring of the GSH level changes within 1 to 10?mM in both directions in living cells. Gel permeation chromatography (GPC) analysis showes that 10% of Real Thiol reacts with thiolated proteins while 90% of Real Thiol reacts with GSH[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Real Thiol (RT) is dissolved in DMSO to obtain a 10 mM stock solutions and further diluted before use.
2. Cells are cultured in 10% FBS DMEM containing different concentrations of Buthionine sulfoximine (0.9, 1.9, 3.9, 7.8, 15.6, 31.3, 62.5, 125, 250 and 500?μM) for 72?h and harvested for analysis.
3. Cells are then suspended in fresh medium containing 1?μM of Real Thiol probe as single-cell suspensions, and keep the staining time between 9 and 10?min for each sample.
4. Analyze sample on a flow cytometer. PE and pacific-blue channels were selected to measure the fluorescence intensities of unreacted and reacted Real Thiol, respectively.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Real Thiol 相关抗体:
分子量

411.36

Formula

C20H17N3O7
CAS 号
性状

固体

颜色

Pink to red

运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

溶解性数据
In Vitro: 

DMSO 中的溶解度 : ≥ 101 mg/mL (245.53 mM; 吸湿的 DMSO 对产品的溶解度有显著影响，请使用新开封的 DMSO)

* "≥" means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.4310 mL 12.1548 mL 24.3096 mL
5 mM 0.4862 mL 2.4310 mL 4.8619 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
纯度 & 产品资料

纯度: 99.87%

COA (264 KB) HNMR (233 KB) LCMS (112 KB)

产品使用指南 (1538 KB)
参考文献
Kinase Assay
[1]

Real Thiol (RT) stock solution is diluted with PBS to the desired concentrations. Equal volumes (typically 20 to 100 μL) of various Glutathione (GSH) solutions with different concentrations are mixed with the Real Thiol solution 10 to 15 min before measurement. All samples are prepared on one 96-well/384-well plate with 3 replicates each. The plate reader is set to read absorption of all samples first. Fluorescent signals are then recorded at λex=405 nm, λem=485 nm, and λex=488 nm, λem=565 nm with bottom read. For testing fluorescent interference by environmental factors, the Real Thiol stock solution is directly diluted with corresponding solutions (for example, certain pH buffer or glycerol solution) and measured using the plate reader with the same settings stated above[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
参考文献

Real Thiol 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.4310 mL 12.1548 mL 24.3096 mL 60.7740 mL
5 mM 0.4862 mL 2.4310 mL 4.8619 mL 12.1548 mL
10 mM 0.2431 mL 1.2155 mL 2.4310 mL 6.0774 mL
15 mM 0.1621 mL 0.8103 mL 1.6206 mL 4.0516 mL
20 mM 0.1215 mL 0.6077 mL 1.2155 mL 3.0387 mL
25 mM 0.0972 mL 0.4862 mL 0.9724 mL 2.4310 mL
30 mM 0.0810 mL 0.4052 mL 0.8103 mL 2.0258 mL
40 mM 0.0608 mL 0.3039 mL 0.6077 mL 1.5194 mL
50 mM 0.0486 mL 0.2431 mL 0.4862 mL 1.2155 mL
60 mM 0.0405 mL 0.2026 mL 0.4052 mL 1.0129 mL
80 mM 0.0304 mL 0.1519 mL 0.3039 mL 0.7597 mL
100 mM 0.0243 mL 0.1215 mL 0.2431 mL 0.6077 mL
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Real Thiol2280796-90-7Fluorescent DyeInhibitorinhibitorinhibit

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