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  3. Sodium 4-phenylbutyrate

Sodium 4-phenylbutyrate  (Synonyms: 苯丁酸钠; 4-PBA sodium; 4-Phenylbutyric acid sodium; Benzenebutyric acid sodium)

目录号: HY-15654 纯度: 99.96%
COA 产品使用指南

Sodium 4-phenylbutyrate (4-PBA sodium) 是一种组蛋白去乙酰化酶 (HDAC) 和内质网应激 (ERS) 抑制剂,可用于癌症和感染等疾病的研究。

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Sodium 4-phenylbutyrate Chemical Structure

Sodium 4-phenylbutyrate Chemical Structure

CAS No. : 1716-12-7

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Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 109 篇科研文献

WB

    Sodium 4-phenylbutyrate purchased from MCE. Usage Cited in: Autophagy. 2021 Nov;17(11):3592-3606.  [Abstract]

    Cells are treated with 20 μM Cannabidiol for 24 h with or without pretreatment with 4-PBA (1 mM for 30 min); expression levels of ER stress and mitophagy related proteins are analyzed by western blotting.

    Sodium 4-phenylbutyrate purchased from MCE. Usage Cited in: Cell Death Dis. 2021 Jun 11;12(6):606.  [Abstract]

    4-PBA administration significantly decreases ER stress-related protein levels of BIP and phospho-eIF2α. Moreover, decreased cleaved caspase 3 and increased occludin and claudin 1 levels are found after 4-PBA treatment.
    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Sodium 4-phenylbutyrate (4-PBA sodium) is an inhibitor of HDAC and endoplasmic reticulum (ER) stress, used in cancer and infection research[1].

    IC50 & Target[1]

    HDAC

     

    体外研究
    (In Vitro)

    Sodium 4-phenylbutyrate (Sodium phenylbutyrate) 是 HDAC 的抑制剂,在 2 mM 时抑制 NSCLC 细胞系的生长。Sodium 4-phenylbutyrate 与 ciglitizone 联合使用可增强癌细胞的生长停滞[1]
    Sodium 4-phenylbutyrate (Sodium phenylbutyrate;0-5 mM) 以剂量依赖的方式抑制 ASFV 感染。Sodium 4-phenylbutyrate 还抑制 ASFV 晚期蛋白质合成并破坏病毒诱导的 H3K9/K14 低乙酰化状态。Sodium 4-phenylbutyrate 和恩诺沙星协同作用以消除 ASFV 复制[2]
    添加 Bafilomycin A1 会导致 LC3II 的积累,而苯丁酸 (4-PBA) 会大大减少这种积累。LPS 会降低 p62 的水平,而苯丁酸会在 LPS 刺激 48 小时后逆转这种降低。具有 LPS 诱导的 AVO 的细胞百分比在 48 小时时增加,而苯丁酸显著降低该百分比。具体而言,苯丁酸处理后具有 AVO 的细胞百分比从 61.6% 降低至 53.1%,支持苯丁酸抑制 LPS 诱导的自噬。作为自噬抑制的阳性对照,使用巴弗洛霉素 A1。巴弗洛霉素 A1 处理可降低具有 LPS 诱导的 AVO 的细胞百分比。在苯丁酸处理后观察到的 OC 面积和融合指数降低在 ATG7 的敲低中没有观察到。使用 BAY 11-7082 和 JSH23 抑制 NF-κB 可降低 LPS 刺激后的 LC3 II 水平,并完全消除苯丁酸对 LPS 诱导作用的抑制作用[3]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    体内研究
    (In Vivo)

    与单独使用 PBS 相比,LPS 会导致显著的骨质流失并降低骨矿物质密度 (BMD)、骨体积 (BV/TV) 和小梁厚度 (Tb. Th),而小梁间隙 (Tb. Sp.) 会增加。Sodium 4-phenylbutyrate (Sodium phenylbutyrate) 可减轻 LPS 诱导的骨质流失。用 Sodium 4-phenylbutyrate 处理可增加 BMD、BV/TV 和 Tb。Th。与单独的 LPS 相比,除了减少 Tb 的扩大。Sp.,但当小鼠单独用 Sodium 4-phenylbutyrate 处理时没有观察到变化。当对 LPS 处理的小鼠施用 Sodium 4-phenylbutyrate 时,通过 TRAP 染色评估的 OC.S/BS 也显著降低。然而,当用 Sodium 4-phenylbutyrate 和 LPS 处理小鼠时,OC.N/BS 趋于降低,尽管没有统计学意义。这些结果表明,Sodium 4-phenylbutyrate 对 LPS 处理小鼠的 OC 的影响是减少其大小而不是数量。与这些发现一致,体内骨吸收的标志物,当向注射 LPS 的小鼠施用 Sodium 4-phenylbutyrate 时,通过 LPS 处理升高的血清 CTX-1 会降低。然而,与单独使用 LPS 相比,与 Sodium 4-phenylbutyrate 共同处理不会显著影响血清 ALP 和骨钙素 (体内骨形成的 2 种标志物) 的水平。Sodium 4-phenylbutyrate 还可降低 LPS 诱导的血清 MCP-1 升高,表明 Sodium 4-phenylbutyrate 可降低 LPS 诱导的全身炎症[3]

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    分子量

    186.18

    Formula

    C10H11NaO2

    CAS 号
    性状

    固体

    颜色

    White to off-white

    中文名称

    苯丁酸钠

    运输条件

    Room temperature in continental US; may vary elsewhere.

    储存方式

    4°C, sealed storage, away from moisture

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    溶解性数据
    In Vitro: 

    H2O 中的溶解度 : 100 mg/mL (537.11 mM; 超声助溶)

    配制储备液
    浓度 溶剂体积 质量 1 mg 5 mg 10 mg
    1 mM 5.3711 mL 26.8557 mL 53.7115 mL
    5 mM 1.0742 mL 5.3711 mL 10.7423 mL
    查看完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    * 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

    • 摩尔计算器

    • 稀释计算器

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    质量
    =
    浓度
    ×
    体积
    ×
    分子量 *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    浓度 (start)

    C1

    ×
    体积 (start)

    V1

    =
    浓度 (final)

    C2

    ×
    体积 (final)

    V2

    In Vivo:

    以下溶解方案,请直接配置工作液。建议现用现配,在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比; 如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶。

    • 方案 一

      请依序添加每种溶剂: PBS

      Solubility: 100 mg/mL (537.11 mM); 澄清溶液; 超声助溶

    动物溶解方案计算器
    请输入动物实验的基本信息:

    给药剂量

    mg/kg

    动物的平均体重

    g

    每只动物的给药体积

    μL

    动物数量

    由于实验过程有损耗,建议您多配一只动物的量
    计算结果
    工作液所需浓度 : mg/mL
    该产品水溶性佳,请具体参考实测 水 / PBS / Saline 中的溶解度数据。
    您所需的储备液浓度超过该产品的实测溶解度,如有需要,请与 MCE 中国技术支持联系。
    纯度 & 产品资料

    纯度: 99.96%

    参考文献
    Cell Assay
    [1]

    Briefly, viable cells, as judged by trypan blue dye exclusion, are seeded at a density of 4 × 104 cells/mL in 60-mm dishes in RPMI 1640 with 10% fetal bovine serum and 0.35% agarose on a base layer of 0.7% agarose. DMSO, TSA, or PB is added to both bottom and top agarose layers. Assays are performed in triplicate on at least three separate occasions, and colonies are counted at 10-14 days[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [3]

    Mice[3]
    Female 10-week-old C57BL/6J mice are housed in the pathogen-free animal facility of IRC. Animals are randomized into the following 4 groups: vehicle control (n=5), vehicle+Benzenebutyric acid (n=6), LPS (n=6), and LPS+Benzenebutyric acid (n=6). Mice are treated with LPS in 200 μL phosphate-buffered saline (PBS) once a week (5 mg/kg, i.p.) for 3 weeks. Sodium 4-phenylbutyrate (Benzenebutyric acid) solution is prepared by titrating equimolecular amounts of Benzenebutyric acid and sodium hydroxide to reach pH 7.4; mice are injected daily intraperitoneally in 200 μL PBS (or with PBS as a vehicle) at a dose of 240 mg/kg for 3 weeks. Mice are sacrificed by CO2 asphyxiation. To determine the bone mineral density (BMD) and microarchitecture of the long bone, the right femur is scanned. Scans are performed with an effective detector pixel size of 6.9 μm and a threshold of 77-255 mg/cc. Trabecular bone is analyzed in a region 1.6 mm in length and located 0.1 mm below the distal femur growth plate[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    参考文献

    完整储备液配制表

    * 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
    储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时,请在6个月内使用,-20°C储存时,请在1个月内使用。

    可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 5.3711 mL 26.8557 mL 53.7115 mL 134.2787 mL
    5 mM 1.0742 mL 5.3711 mL 10.7423 mL 26.8557 mL
    10 mM 0.5371 mL 2.6856 mL 5.3711 mL 13.4279 mL
    15 mM 0.3581 mL 1.7904 mL 3.5808 mL 8.9519 mL
    20 mM 0.2686 mL 1.3428 mL 2.6856 mL 6.7139 mL
    25 mM 0.2148 mL 1.0742 mL 2.1485 mL 5.3711 mL
    30 mM 0.1790 mL 0.8952 mL 1.7904 mL 4.4760 mL
    40 mM 0.1343 mL 0.6714 mL 1.3428 mL 3.3570 mL
    50 mM 0.1074 mL 0.5371 mL 1.0742 mL 2.6856 mL
    60 mM 0.0895 mL 0.4476 mL 0.8952 mL 2.2380 mL
    80 mM 0.0671 mL 0.3357 mL 0.6714 mL 1.6785 mL
    100 mM 0.0537 mL 0.2686 mL 0.5371 mL 1.3428 mL

    * 备注:如您选择水作为储备液,请稀释至工作液后,再用 0.22 μm 的滤膜过滤除菌后使用。

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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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