1. Cell Cycle/DNA Damage
  2. DNA/RNA Synthesis

DNA/RNA Synthesis

RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.

Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.

First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.

DNA/RNA Synthesis 相关产品 (57):

Cat. No. Product Name Effect Purity
  • HY-12320
    Cycloheximide Inhibitor 99.45%
    Cycloheximide (Naramycin A) 是真核生物蛋白质合成的抑制剂,抑制体内蛋白质合成和RNA合成的 IC50 值分别为532.5 nM 和 2880 nM。
  • HY-17559
    Actinomycin D Inhibitor 99.89%
    Actinomycin D 抑制 DNA 修复,IC50 为 0.42 μM。
  • HY-13316
    Mitomycin C Inhibitor 98.89%
    Mitomycin C是一种抗肿瘤药物和抗生素,可有效的抑制DNA合成 (DNA synthesis)。
  • HY-17026
    Gemcitabine Inhibitor 99.80%
    Gemcitabine (NSC 613327;LY188011) 是一种 DNA合成 抑制剂,抑制 BxPC-3,Mia Paca-2,PANC-1,PL-45 和 AsPC-1细胞生长的 IC50 分别为 37.6, 42.9, 92.7, 89.3 和 131.4 nM。
  • HY-19610
    alpha-Amanitin Inhibitor 99.79%
    alpha-Amanitin是几种致命毒蘑菇的主要毒素,通过抑制 RNA聚合酶II 发挥其毒性作用。
  • HY-13753
    Streptozocin Inhibitor 99.58%
    Streptozocin是一种有效的 DNA甲基化 试剂,在HL60,K562和C1498细胞中的IC50 分别为11.7,904 和 1024 μg/mL。
  • HY-17565
    Bleomycin sulfate Inhibitor >98.0%
    Bleomycin sulfate是具有有效抗肿瘤活性的DNA合成抑制剂。
  • HY-B0003
    Gemcitabine Hydrochloride Inhibitor 99.93%
    Gemcitabine hydrochloride是一种DNA合成抑制剂,在131.4 nM -2,PANC-1,PL-45和AsPC-1细胞中的 IC50 分别为37.6,42.9,92.7,89.3 和 131.4 nM。
  • HY-18982
    Anisomycin Inhibitor 98.03%
    Anisomycin是一种有效的蛋白质合成抑制剂,它通过抑制肽基转移酶或80S核糖体系统干扰蛋白质和DNA合成
  • HY-17393
    Carboplatin Inhibitor 99.88%
    Carboplatin是一种 DNA 合成 抑制剂,其与DNA结合,抑制复制和转录并诱导细胞死亡。 卡铂是顺铂的衍生物,是一种有效的抗癌剂。
  • HY-12742
    SCR7 Inhibitor 99.75%
    SCR7是具有抗癌活性的DNA连接酶IV抑制剂。
  • HY-13323
    CX-5461 Inhibitor 98.47%
    CX-5461是一种有效,口服的rRNA合成抑制剂。 它在HCT-116,A375和MIA PaCa-2细胞中抑制RNA聚合酶I驱动的rRNA转录,IC50为142,113和54 nM。
  • HY-B0016
    Capecitabine Inhibitor 99.97%
    Capecitabine 是一种可用于口服的前药,可由胸苷磷酸化酶催化转变为其活性代谢物 Fluorouracil (FU)。
  • HY-19620
    LMI070 Agonist 98.78%
    LMI070 (Branaplam) 是一种高效,选择性和口服活性的小分子 SMN2 剪接调节剂。
  • HY-19931
    COH29 Inhibitor 98.04%
    COH29是一种高效,具有抗癌活性的核糖核苷酸还原酶 (RNR) 抑制剂。抑制核糖核苷酸还原酶的 IC50 为16 μM。
  • HY-B0313
    Hydroxyurea Inhibitor >98.0%
    Hydroxyurea是通过抑制核糖核苷酸还原酶抑制DNA合成的细胞凋亡诱导剂。
  • HY-15457
    Triciribine Inhibitor 99.20%
    Triciribine 是一种 DNA 合成 (DNA synthesis) 抑制剂,也抑制 AktHIV-1/2IC50 分别为 130 nM 和 0.02-0.46 μM。
  • HY-16637
    Folic acid 99.56%
    Folic acid(Vitamin M; Vitamin B9)是维生素B9。
  • HY-101792
    RG7800 Modulator 99.86%
    RG7800是一种小分子SMN2剪接修饰剂,进入人体临床试验用于治疗脊髓性肌萎缩。
  • HY-12342
    ML216 Inhibitor
    ML216(CID-49852229)是一BLM解螺旋酶有效抑制剂,对BLM full length和BLM636–1298的IC50值分别为3.0uM和0.97uM。
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