1. Cell Cycle/DNA Damage
  2. DNA/RNA Synthesis

DNA/RNA Synthesis

RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.

Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.

First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.

DNA/RNA Synthesis 相关产品 (56):

Cat. No. Product Name Effect Purity
  • HY-12320
    Cycloheximide Inhibitor 99.45%
    Cycloheximide (Naramycin A;Actidione) 是真核生物 蛋白质合成 的抑制剂,抑制体内蛋白质合成和RNA合成的 IC50 值分别为532.5 nM 和 2880 nM。
  • HY-17559
    Actinomycin D Inhibitor 99.89%
    Actinomycin D 抑制DNA修复,IC50 为 0.42 μM。
  • HY-13316
    Mitomycin C Inhibitor 98.89%
    Mitomycin C 是一种DNA损伤剂,能抑制DNA合成并诱导凋亡。
  • HY-17026
    Gemcitabine Inhibitor 99.80%
    Gemcitabine 是一种 DNA synthesis 抑制剂,作用于 BxPC-3,Mia Paca-2,PANC-1,PL-45 和 AsPC-1 细胞,IC50 分别为 37.6,42.9,92.7,89.3 和 131.4 nM。
  • HY-19610
    alpha-Amanitin Inhibitor 99.79%
    alpha-Amanitin 是一些剧毒蘑菇中的主要毒素,通过抑制 RNA-polymerase II 来发挥其毒性功能。
  • HY-13753
    Streptozocin Inhibitor 99.58%
    Streptozocin 是一种有效的 DNA 甲基化试剂,作用于 HL60,K562 和 C1498 细胞,IC50 分别为 11.7,904 和 1024 μg/mL。
  • HY-18982
    Anisomycin Inhibitor 98.03%
    Anisomycin 是一种有效的 JNK 激活剂。
  • HY-B0003
    Gemcitabine Hydrochloride Inhibitor 99.93%
    Gemcitabine hydrochloride 是一种 DNA synthesis 抑制剂,作用于 BxPC-3,Mia Paca-2,PANC-1,PL-45 和 AsPC-1 细胞,IC50 分别为 37.6,42.9,92.7,89.3 和 131.4 nM。
  • HY-17565
    Bleomycin sulfate Inhibitor >98.0%
    Bleomycin sulfate 是一种有效的DNA损伤剂,用作微核 (micronucleus,MN) 诱导剂。Bleomycin 也是一种天然抗生素。
  • HY-17393
    Carboplatin Inhibitor 98.25%
    Carboplatin 是一种抗肿瘤剂,可以与 DNA 结合,干扰 DNA 复制。
  • HY-12742
    SCR7 Inhibitor 99.75%
    SCR7 是一种DNA连接酶IV (DNA Ligase IV) 抑制剂,抑制非同源末端连接 (NHEJ)。SCR7 增强CRISPR/Cas9条件的编辑频率。
  • HY-13323
    CX-5461 Inhibitor 98.47%
    CX-5461 是一种有效的,可口服的 Pol I 介导的 rRNA synthesis 抑制剂,在 HCT-116,A375 和 MIA PaCa-2 细胞中,IC50 值分别为 142 nM,113 nM 和 54 nM,对 Pol II 作用不大 (IC50 ≥25 μM)。
  • HY-19620
    LMI070 Agonist 98.78%
    LMI070 (NVS-SM1) 是一种RNA剪接增强剂, 用于治疗脊髓性肌萎缩症。
  • HY-B0016
    Capecitabine Inhibitor 99.97%
    Capecitabine 是一种可用于口服的前药,可由胸苷磷酸化酶催化转变为其活性代谢物 Fluorouracil (FU)。
  • HY-B0313
    Hydroxyurea Inhibitor >98.0%
  • HY-19931
    COH29 Inhibitor 98.04%
    COH29是一种高效的RNR抑制剂,具有抗癌活性, 在KB细胞中的IC50值为8 μM。
  • HY-15457
    Triciribine Inhibitor 99.20%
    Triciribine 是一种 DNA 合成 (DNA synthesis) 抑制剂,也抑制 AktHIV-1/2IC50 分别为 130 nM 和 0.02-0.46 μM。
  • HY-16637
    Folic acid >98.0%
    Folic acid(Vitamin M; Vitamin B9)是维生素B9。
  • HY-101792
    RG7800 Modulator 99.86%
  • HY-12342
    ML216 Inhibitor
    ML216(CID-49852229)是一BLM解螺旋酶有效抑制剂,对BLM full length和BLM636–1298的IC50值分别为3.0uM和0.97uM。
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