1. Cell Cycle/DNA Damage
  2. DNA/RNA Synthesis

DNA/RNA Synthesis

RNA synthesis, which is also called DNA transcription, is a highly selective process. Transcription by RNA polymerase II extends beyond RNA synthesis, towards a more active role in mRNA maturation, surveillance and export to the cytoplasm.

Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA.DNA ligases discriminate against substrates containing RNA strands or mismatched base pairs at positions near the ends of the nickedDNA. Bleomycin (BLM) exerts its genotoxicity by generating free radicals, whichattack C-4′ in the deoxyribose backbone of DNA, leading to opening of the ribose ring and strand breakage; it is an S-independentradiomimetic agent that causes double-strand breaks in DNA.

First strand cDNA is synthesized using random hexamer primers and M-MuLV Reverse Transcriptase (RNase H). Second strand cDNA synthesis is subsequently performed using DNA Polymerase I and RNase H. The remaining overhangs are converted into blunt ends using exonuclease/polymerase activity. After adenylation of the 3′ ends of DNA fragments, NEBNext Adaptor with hairpin loop structure is ligated to prepare the samples for hybridization. Cell cycle and DNA replication are the top two pathways regulated by BET bromodomain inhibition. Cycloheximide blocks the translation of mRNA to protein.

DNA/RNA Synthesis 相关产品 (37):

Cat. No. Product Name Effect Purity
  • HY-12320
    Cycloheximide Inhibitor 99.17%
    Cycloheximide 是真核生物中蛋白质生物合成的抑制剂,抑制蛋白合成和RNA合成,IC50 分别为 532.5 nM 和 2880 nM。
  • HY-17559
    Actinomycin D Inhibitor 99.89%
    Actinomycin D 抑制DNA修复,IC50 为 0.42 μM。
  • HY-13316
    Mitomycin C Inhibitor 98.51%
    Mitomycin C 是一种DNA损伤剂,能抑制DNA合成并诱导凋亡。
  • HY-17026
    Gemcitabine Inhibitor >98.0%
    Gemcitabine 是一种 DNA synthesis 抑制剂,作用于 BxPC-3,Mia Paca-2,PANC-1,PL-45 和 AsPC-1 细胞,IC50 分别为 37.6,42.9,92.7,89.3 和 131.4 nM。
  • HY-12742
    SCR7 Inhibitor 99.78%
    SCR7 是一种DNA连接酶IV (DNA Ligase IV) 抑制剂,抑制非同源末端连接 (NHEJ)。SCR7 增强CRISPR/Cas9条件的编辑频率。
  • HY-101792
    RG7800 Modulator 98.22%
    RG7800是一种小分子SMN2剪接修饰剂,进入人体临床试验用于治疗脊髓性肌萎缩。
  • HY-U00337
    Datelliptium chloride Inhibitor
    Datelliptium chloride 是一种源于 ellipticine 的 DNA 嵌合剂,具有抗肿瘤活性。
  • HY-19610
    alpha-Amanitin Inhibitor
    alpha-Amanitin 是一些剧毒蘑菇中的主要毒素,通过抑制 RNA-polymerase II 来发挥其毒性功能。
  • HY-13753
    Streptozocin Inhibitor 99.58%
    Streptozocin 是一种有效的 DNA 甲基化试剂,作用于 HL60,K562 和 C1498 细胞,IC50 分别为 11.7,904 和 1024 μg/mL。
  • HY-B0003
    Gemcitabine Hydrochloride Inhibitor 99.62%
    Gemcitabine hydrochloride 是一种 DNA synthesis 抑制剂,作用于 BxPC-3,Mia Paca-2,PANC-1,PL-45 和 AsPC-1 细胞,IC50 分别为 37.6,42.9,92.7,89.3 和 131.4 nM。
  • HY-B0016
    Capecitabine Inhibitor 99.68%
    Capecitabine 是一种可用于口服的前药,可由胸苷磷酸化酶催化转变为其活性代谢物 Fluorouracil (FU)。
  • HY-17565
    Bleomycin sulfate Inhibitor >98.0%
    Bleomycin sulfate 是一种有效的DNA损伤剂,用作微核 (micronucleus,MN) 诱导剂。Bleomycin 也是一种天然抗生素。
  • HY-13323
    CX-5461 Inhibitor 98.47%
    CX-5461 是一种 rRNA synthesis 抑制剂,在 HCT-116,A375 和 MIA PaCa-2 细胞中,抑制Pol驱动的rRNA转录,IC50 值为 142 nM,对 Pol II 没有作用。
  • HY-B0313
    Hydroxyurea Inhibitor >98.0%
    Hydroxyurea是通过抑制核糖核苷酸还原酶抑制DNA合成的细胞凋亡诱导剂。
  • HY-15457
    Triciribine Inhibitor 99.20%
    Triciribine 是一种 DNA 合成 (DNA synthesis)抑制剂,也抑制 AktHIV-1/2IC50 分别为 130 nM 和 0.02-0.46 μM。
  • HY-19620
    LMI070 Agonist 98.78%
    LMI070 (NVS-SM1) 是一种RNA剪接增强剂, 用于治疗脊髓性肌萎缩症。
  • HY-19931
    COH29 Inhibitor 98.04%
    COH29是一种高效的RNR抑制剂,具有抗癌活性, 在KB细胞中的IC50值为8 μM。
  • HY-12814
    TH588 Inhibitor 98.52%
    TH588是MTH1(NUDT1)高效选择性抑制剂,IC50值5nM。
  • HY-16965
    TH287 Inhibitor 98.11%
    TH287是MTH1 (NUDT1)高效选择性抑制剂,IC50值0.8nM,对MTH2, NUDT5, NUDT12, NUDT14等抑制力弱。
  • HY-10082
    Triapine Inhibitor 98.32%
    Triapine 是一种核糖核苷酸还原酶 (ribonucleotide reductaseRR)的M2 亚基抑制剂,是有效的放射致敏剂。