2. JAK/STAT Signaling Protein Tyrosine Kinase/RTK Antibody-drug Conjugate/ADC Related
  3. Radionuclide-Drug Conjugates (RDCs) EGFR ADC Antibody
  4. Trastuzumab

Trastuzumab  (Synonyms: 曲妥珠单抗; Anti-Human HER2, Humanized Antibody)

目录号: HY-P9907 纯度: 99.80%
COA 技术支持

Trastuzumab 是一种人源化 IgG1 单克隆抗体，其以高亲和力与 HER2 选择性结合。Trastuzumab 可用于 HER2 阳性转移性乳腺癌和胃癌的研究。(Note: 下方产品规格仅指示 Trastuzumab 有效含量，该产品成分比例为 Trastuzumab : 辅料 = 44 : 78。)

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CAS No. : 180288-69-1

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规格 价格 是否有货 数量
1 mg ￥1600
In-stock
5 mg ￥5500
In-stock
10 mg ￥7400
In-stock
50 mg   询价  
100 mg   询价  

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Customer Review

Other Forms of Trastuzumab:

Trastuzumab 相关抗体

Top Publications Citing Use of Products

MCE 顾客使用本产品发表的 40 篇科研文献

Cell Viability Assay

    Trastuzumab purchased from MCE. Usage Cited in: Nat Commun. 2023 Sep 14;14(1):5699.

    Trastuzumab (TZB) (3.16-1000 nM; 14 h) is cytotoxic to GBC-SD and EH-GB1 cells.

    查看 Radionuclide-Drug Conjugates (RDCs) 亚型特异性产品:

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    RDC Peptide RDC Antibody RDC Bifunctional Chelator RDC Linker Other

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    EGFR/ErbB1/HER1 ErbB2/HER2 ErbB3/HER3 ErbB4/HER4

    • 生物活性

    • 实验参考方法

    • 纯度 & 产品资料

    • 参考文献

    生物活性

    Trastuzumab is a humanized IgG1 monoclonal antibody that selectively binds to HER2 with high affinity. Trastuzumab can be used for the research of HER2-positive metastatic breast cancer and gastric cancer[1][2][3]. (Note: The product specifications below only indicate the effective content of Trastuzumab. The component ratio of this product is Trastuzumab : excipients = 44 : 78.)

    同型

    Human IgG1 kappa
    推荐同型对照抗体
    IC50 & Target[1]

    RDC Antibody

     

    HER2

     

    细胞效力
    (Cellular Effect)
    Cell Line Type Value Description References
    MOLT-4 IC50
    3.25 μM
    Compound: 1a
    Cytotoxic effect on the growth of human T-and B-lymphoblastoid cell lines(MOLT-4 and MGL-8) evaluated by measuring inhibition of thymidine intake in presence of 10 uM deoxyguanosine
    Cytotoxic effect on the growth of human T-and B-lymphoblastoid cell lines(MOLT-4 and MGL-8) evaluated by measuring inhibition of thymidine intake in presence of 10 uM deoxyguanosine
    		[PMID: 1573639]
    体外研究
    (In Vitro)

    用 Trastuzumab 处理过表达 HER2 的乳腺癌细胞系会导致 p27KIP1 和 Rb 相关蛋白 p130 的诱导，从而显著减少经历 S 期的细胞数量。由于 Trastuzumab 与 HER2 过表达细胞结合，在体外观察到许多其他表型变化。Trastuzumab 通过其人免疫球蛋白 G1 Fc 结构域与人体免疫系统相互作用可能会增强其抗肿瘤活性。体外研究表明，Trastuzumab 在介导抗体依赖性细胞介导的针对 HER2 过表达肿瘤靶标的细胞毒性方面非常有效[1]。Trastuzumab 由两个抗原特异性位点组成，它们结合 HER2 受体胞外结构域的近膜部分，并阻止其胞内酪氨酸激酶的激活。Trastuzumab 募集负责抗体依赖性细胞毒性的免疫效应细胞[2]。Trastuzumab IgG 的存在显著增加了对所有乳腺癌细胞系的杀伤力。Trastuzumab 诱发的 PBMC 的 ADCC 活性对 Trastuzumab 敏感 (SKBR-3) 或 Trastuzumab 耐药 (JIMT-1) 乳腺癌细胞同样很强，剂量依赖性细胞死亡在效应物/靶标处达到 50-60%，杀死比例为 60:1[3]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Trastuzumab 相关抗体:
    体内研究
    (In Vivo)

    小鼠异种移植模型的 Trastuzumab 处理可显著抑制肿瘤生长。当与标准细胞毒性化疗药物联合使用时，Trastuzumab 处理与单独使用任一药物相比通常具有统计学上更好的抗肿瘤疗效[1]。Trastuzumab 可显著抑制裸鼠和 SCID 小鼠肉眼可见的 JIMT-1 异种移植肿瘤的生长[3]。

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Clinical Trial
    基因 ID

    2064  [NCBI]

    Accession
    应用

    ELISA, FACS, Functional assay
    偶联物

    Unconjugated
    复溶方法

    The product can be reconstituted/diluted with sterile PBS or saline.
    分子量

    145.36 kDa

    CAS 号
    性状

    固体

    颜色

    White to light yellow

    中文名称

    曲妥珠单抗；曲妥单抗；群司珠单抗
    储存方式

    Please store the product under the recommended conditions in the Certificate of Analysis.
    Format
    • Human IgG1 kappa
    Biological Activity
    • Loaded Trastuzumab on AHC2 biosensor, can bind Her2/CD340 Protein, Human (Biotinylated, HEK293, His, HY-P78882) with an affinity constant of 6.903E-10 M as determined in BLI assay.
    • Trastuzumab has a significant ADCC effect on BT474 cells in a dose-dependent manner.The EC50 for this effect is 26.36 and 32.09 ng/mL.
    • Trastuzumab has a significant ADCC effect on SK-BR-3 cells in a dose-dependent manner.The EC50 for this effect is 14.43 and 16.70 ng/mL.
    • Immobilized HER2/CD340 Protein, Human (630a.a, HEK293, His, HY-P70254) can bind Trastuzumab.The EC50 for this effect is 2.16 ng/mL.
    纯度 & 产品资料

    纯度: 99.80%

    COA (265 KB)

    抗体抑制剂使用指南 (1152 KB)
    参考文献
    Cell Assay
    [3]

    The effects of Trastuzumab and Trastuzumab-F(ab′)2 on the growth of JIMT-1, SKBR-3, and BT-474 cells are evaluated using the AlamarBlue method. Exponentially growing cells are harvested and plated in single wells of a 96-well flat-bottomed tissue culture plate at defined densities, ranging from 4,500-8,000 cells per well depending on the cell line. After overnight culture, the regular medium is exchanged to medium containing 0, 1, 10, or 100 μg/mL Trastuzumab or Trastuzumab-F(ab′) 2. Cell viability is tested after 72 h of treatment. Fluorescence is detected at an excitation of 544 nm, and emission is detected at 590 nm[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [3]

    Trastuzumab and Trastuzumab-F(ab′)2 are given at a dose of 5 and 25 μg/g, respectively, by weekly i.p. injection. The five times greater amount of administered F(ab′)2 is chosen based on the different half-lives of IgG and F(ab′) F(ab′)2. Control mice are treated with weekly i.p. injection of 100 μL physiologic saline (saline). Animals are euthanized by CO2 inhalation[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    参考文献

    Trastuzumab 相关分类

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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Trastuzumab180288-69-1Anti-Human HER2, Humanized Antibody曲妥珠单抗曲妥单抗群司珠单抗Radionuclide-Drug Conjugates (RDCs)EGFRADC AntibodyEpidermal growth factor receptorErbB-1HER1Inhibitorinhibitorinhibit

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