AMPK beta 1 抗体

AMPK beta 1 Antibody is a non-conjugated and Rabbit origined polyclonal antibody about 30 kDa, targeting to AMPK beta 1. It can be used for WB,IHC-P,FC,IP assays with tag free, in the background of Human, Mouse, Rat.

AMPK beta 1：该基因编码的蛋白质是 AMP 活化蛋白激酶 (AMPK) 的调节亚基。AMPK 是一种异源三聚体，由一个 α 催化亚基以及非催化 β 和 γ 亚基组成。AMPK 是一种重要的能量感应酶，可监测细胞能量状态。响应细胞代谢应激，AMPK 被激活，从而使乙酰辅酶 A 羧化酶 (ACC) 和 β-羟基β-甲基戊二酰辅酶 A 还原酶 (HMGCR) 磷酸化和失活，这些酶是参与调节脂肪酸和胆固醇从头生物合成的关键酶.该亚基可能是 AMPK 活性的正调节因子。该亚基的豆蔻酰化和磷酸化已被证明会影响 AMPK 的酶活性和细胞定位。该亚基也可作为衔接分子介导 AMPK 复合物的结合。[由 RefSeq 提供，2008 年 7 月]

Free

5564

Predicted band size: 30 kDa;Observed band size: 40 kDa

affinity purified

Non-conjugated

Signal Transduction

Primary Antibody; Rabbit Polyclonal Antibody

Rabbit

Human, Mouse, Rat

Q9Y478

WB: 1:500-1:1,000 ;IHC: 1:50-1:100 ;IP: 1:20 ;FC: 1:50-1:100

• 
  Western blot analysis of extracts from NIH-3T3 (lane 2(20μg) or lane 3(40μg)) and Hela(lane 4(20μg) or lane 5(40μg)), using AMPK beta 1 (HY-P80542) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80542, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (HY-P8004/HY-P8001, 1/10,000) was used for 1 hour at room temperature.

WB, IHC-P, FC, IP

液体

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Synthetic peptide corresponding to Human AMPK beta 1.The exact sequence is proprietary to MCE.