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  5. FOXP3 抗体 (YA425)

FOXP3 抗体 (YA425)

目录号: HY-P80135
COA 抗体使用指南 技术支持

FOXP3 Antibody 是一个非偶联、分子量约 47 kDa、兔源、抗 FOXP3 单克隆抗体。它可用于人背景下 WB、ICC、FC 实验，且不带标记。

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规格 价格 是否有货 数量
10 μL ￥470 In-stock
50 μL ￥1220 In-stock
100 μL ￥2000 In-stock
250 μL   询价  

* Please select Quantity before adding items.

Top Publications Citing Use of Products

【WB: 蛋白质免疫印迹; IHC-P: 石蜡切片样本的免疫组织化学; IHC-F: 冰冻切片样本的免疫组织化学; ICC/IF: 细胞免疫荧光; IF-Tissue: 组织免疫荧光; mIHC: 多重荧光免疫组化; IP: 免疫沉淀; ChIP: 染色质免疫沉淀; FC: 流式细胞术; ELISA: 酶联免疫吸附试验】

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  • 技术参数

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描述

FOXP3 Antibody is a non-conjugated and Rabbit origined monoclonal antibody about 47 kDa, targeting to FOXP3. It can be used for WB,ICC,FC assays with tag free, in the background of Human.
标签

Free
基因 ID
蛋白数据库
中文名
FOXP3 抗体
分子量

Predicted band size: 47 kDa
纯度

Protein A affinity purified.
亚细胞定位

Nucleus, Cytoplasm.
偶联

Non-conjugated
修饰

Unmodified
RRID
研究领域

Cell Biology
产品类别

Primary Antibody; Recombinant Rabbit Monoclonal Antibody
克隆性

Recombinant, Monoclonal
宿主

Rabbit
反应物种

Human
推荐稀释比例

WB: 1:500-1:2,000 ;ICC: 1:50-1:100 ;FC: 1:50-1:100

  • Western blot analysis of extracts from K562 (lane 1) and Raji (lane 2) using FOXP3 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% nonfat powdered milk in PBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, 1/3000) was diluted with 5% nonfat powdered milk in PBST at 4°C overnight. Goat Anti-Rabbit IgG-HRP Secondary Antibody (1/8,000) was incubated for 45min at room temperature.
应用

WB, ICC/IF, FC
性状

液体
组分

Supplied in 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.
保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
运输条件

Shipping with blue ice.
同型

IgG
敏感性

Endogenous
免疫原

Synthetic peptide corresponding to Human FOXP3.AA range:250-431.
数据库

Entrez Gene: 50943 Human

SwissProt: Q9BZS1 Human

OMIM: 304790 Human

文件资料

COA (193 KB)

抗体使用指南 (1083 KB)

FOXP3 Antibody (YA425) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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目录号：
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