IL-1 beta 抗体

IL-1 beta Antibody is a non-conjugated and Rabbit origined polyclonal antibody about 17 kDa, targeting to IL-1 beta. It can be used for WB,IHC-P,ICC/IF assays with tag free, in the background of Human, Mouse, Rat.

IL-1 beta：该基因编码的蛋白质是白介素 1 细胞因子家族的成员。这种细胞因子由活化的巨噬细胞产生，作为前蛋白，通过半胱天冬酶 1 (CASP1/ICE) 蛋白水解加工成其活性形式。这种细胞因子是炎症反应的重要介质，参与多种细胞活动，包括细胞增殖、分化和凋亡。发现这种细胞因子在中枢神经系统 (CNS) 中诱导环氧合酶 2 (PTGS2/COX2) 会导致炎症性疼痛超敏反应。同样，IL-1B 与人类骨关节炎的发病机制有关。2019 年严重冠状病毒病 (COVID-19) 患者的支气管肺泡灌洗液样本中的促炎细胞因子水平升高，例如 IL-1B。严重急性呼吸系统综合症冠状病毒2（SARS-CoV-2）引起的肺损伤在很大程度上是IL-1B等细胞因子促进炎症反应的结果。该基因和其他八个白细胞介素 1 家族基因在 2 号染色体上形成一个细胞因子基因簇。[由 RefSeq 提供，2020 年 7 月]

Free

P01584

Predicted band size: 17 kDa;Observed band size: 17 kDa

affinity purified

Non-conjugated

Unmodified

AB_3102173

Immunology

Primary Antibody; Rabbit Polyclonal Antibody

Polyclonal

Rabbit

Human, Mouse, Rat

WB: 1:500-1:1,000 ;IHC: 1:50-1:100 ;IF: 1:50-1:200

• 
  Western blot analysis of extracts from A431(lane 2(20μg), RAW264.7 (lane 3(20μg) and A549(lane 4(20μg) using IL-1 beta (HY-P80720) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using IL-1 beta Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80720, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded Mouse colon tissue using IL-1 beta Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P80720, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P, ICC/IF

液体

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG

Endogenous

Synthetic peptide corresponding to Human IL1B AA range 181-230.