1. 抗体
  2. 一抗
  3. 单克隆抗体 流式抗体
  4. JAK2 抗体 (YA721)

JAK2 抗体 (YA721)

目录号: HY-P80421
COA 抗体使用指南 技术支持

JAK2 Antibody (YA721) 是一个非偶联、分子量约 130kDa、小鼠来源的抗 JAK2 单克隆抗体。它可用于人、小鼠、大鼠背景下 WB、ICC、IHC-P、FC 实验,且不带标记。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 μL ¥470 In-stock
50 μL ¥1220 In-stock
100 μL ¥2000 In-stock
250 μL   询价  

* Please select Quantity before adding items.

【WB: 蛋白质免疫印迹; IHC-P: 石蜡切片样本的免疫组织化学; IHC-F: 冰冻切片样本的免疫组织化学; ICC/IF: 细胞免疫荧光; IF-Tissue: 组织免疫荧光; mIHC: 多重荧光免疫组化; IP: 免疫沉淀; ChIP: 染色质免疫沉淀; FC: 流式细胞术; ELISA: 酶联免疫吸附试验】

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

JAK2 Antibody (YA721) is a non-conjugated and Mouse origined monoclonal antibody about 130kDa, targeting to JAK2. It can be used for WB,ICC,IHC-P,FC assays with tag free, in the background of Human, Mouse, Rat.

标签

Free

基因 ID
蛋白数据库
中文名
JAK2 抗体 (YA721)
分子量

Predicted band size: 130kDa; Observed band size: 130 kDa

纯度

Protein A affinity purified.

亚细胞定位

Cytopasm, nucleus

偶联

Non-conjugated

修饰

Unmodified

RRID
研究领域

Cell Biology

产品类别

Primary Antibody; Mouse Monoclonal Antibody

克隆性

Monoclonal

宿主

Mouse

反应物种

Human, Mouse, Rat

推荐稀释比例

WB: 1:2,000 ;ICC: 1:50 ;FC: 1:200 ;IHC-P: 1:200

  • Western blot analysis of extracts from K562 (lane 2(20μg), RAW264.7 (lane 3(20μg) and C6(lane 4(40μg) using JAK2 (HY-P80421) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
  • Immunocytochemistry analysis of RAW264.7 cells labeling JAK2 with JAK2 Antibody (HY-P80421)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with JAK2 Antibody (HY-P80421) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L(HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
  • Immunocytochemistry analysis of MCF-7 cells labeling JAK2 with JAK2 Antibody (HY-P80421) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with JAK2 Antibody (HY-P80421) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Mouse IgG H&L(HY-P8005, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
应用

WB, ICC/IF, IHC-P, FC

性状

液体

组分

Supplied in 1*PBS (pH7.4), 0.2% BSA and 40% Glycerol. Preservative: 0.05% Sodium Azide.

保存条件 & 期限

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

运输条件

Shipping with blue ice.

同型

IgG2b

敏感性

Endogenous

免疫原

Synthetic peptide corresponding to Human JAK2.AA range:883-1132.

数据库
文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
JAK2 Antibody (YA721)
目录号:
HY-P80421
需求量: