PI3 Kinase p85 alpha 抗体 (YA689)

PI3 Kinase p85 alpha Antibody (YA689) is a non-conjugated and Mouse origined monoclonal antibody about 84 kDa, targeting to PI3 Kinase p85 alpha (1C8). It can be used for WB,IHC-P assays with tag free, in the background of Human, Mouse, Rat.

PI3 Kinase p85 alpha (1C8)：磷脂酰肌醇 3-激酶在 3-素数位置磷酸化磷脂酰肌醇的肌醇环。该酶包含一个 110 kD 的催化亚基和一个 85、55 或 50 kD 的调节亚基。该基因编码 85 kD 调节亚基。磷脂酰肌醇 3-激酶在胰岛素的代谢作用中起着重要作用，该基因的突变与胰岛素抵抗有关。该基因的可变剪接导致编码不同亚型的四种转录变体。[由 RefSeq 提供，2011 年 6 月]

Free

P27986

Predicted band size: 84 kDa

affinity purified

Non-conjugated

Unmodified

AB_3102237

Signal Transduction

Primary Antibody; Mouse Monoclonal Antibody

Monoclonal

Mouse

Human, Mouse, Rat

WB: 1:500-1:1,000 ;IHC: 1:50-1:100

• 
  Immunohistochemical analysis of paraffin-embedded rat liver tissue using PI3 Kinase p85 alpha Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
• 
  Immunohistochemical analysis of paraffin-embedded rat liver tissue using PI3 Kinase p85 alpha Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

WB, IHC-P

液体

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping with blue ice.

IgG1

Endogenous