Acetylcholinesterase/ACHE 蛋白, Mouse (HEK293, His)

Acetylcholinesterase (ACHE) proteins exhibit multiple functions, including acetylcholinesterase activity and protein self-association. It regulates acetylcholine processing, receptor signaling and recycling. Acetylcholinesterase/ACHE Protein, Mouse (HEK293, His) is the recombinant mouse-derived Acetylcholinesterase/ACHE protein, expressed by HEK293 , with C-10*His labeled tag.

乙酰胆碱酯酶 (ACHE) 蛋白具有多种功能，表现出乙酰胆碱酯酶活性、相同的蛋白结合活性以及蛋白自缔合能力。它参与冷诱导产热的积极调节，使其处于关键过程的上游，例如乙酰胆碱分解代谢过程、乙酰胆碱受体信号传导途径和受体再循环的调节。ACHE 存在于多种细胞成分中，包括基底膜、细胞表面和神经肌肉接头。它的表达跨越多个结构，包括消化系统、泌尿生殖系统、肌肉组织、神经系统和感觉器官。值得注意的是，ACHE 的人类直系同源物与阿尔茨海默病、癫痫和重症肌无力等重要健康状况有关，凸显了该蛋白质在各种生理环境中的重要性。广泛的表达模式进一步强调了其在多种组织中的基本作用，特别是在小脑和胸腺中。

Measured by its ability to cleave Acetylthiocholine. The specific activity is > 250 nmoL/min/μg, as measured under the described conditions.

Materials
Assay buffer: 0.1 M sodium phosphate with 0.05% Brij-35, pH 7.5
Acetylcholinesterase/ACHE Protein, Mouse (HEK293, His) (HY-P72811)
DTNB (HY-15915): Dissolve in DMSO to make a 10 mM solution, then dilute with buffer to 100 μM
Substrate mixture: First dilute ATC in DMSO to 20 mM, then dilute ATC to 200 μM with 100 μM DTNB to prepare the substrate mixture

Assay Procedure
1. Dilute Mouse ACHE protein to 0.02 μg/mL with buffer;
2. Add 50 μL of 0.02 μg/mL Mouse ACHE protein buffer to a 96-well plate, then add 50 μL of substrate to start the reaction. Include three replicates per group and a blank control containing 50 μL of buffer and 50 μL of substrate mixture.
3. Zero the microplate reader using the OD value of the blank group, and read the absorbance at 405 nm in kinetic mode for 5 minutes.
4. Calculate the specific activity:

Mouse

HEK293

C-10*His

P21836-1/NP_033729.1 (E32-L614)

11423  [NCBI]

Full Length of Isoform-1 Mature Protein

EGREDPQLLVRVRGGQLRGIRLKAPGGPVSAFLGIPFAEPPVGSRRFMPPEPKRPWSGVLDATTFQNVCYQYVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPASPTPVLIWIYGGGFYSGAASLDVYDGRFLAQVEGAVLVSMNYRVGTFGFLALPGSREAPGNVGLLDQRLALQWVQENIAAFGGDPMSVTLFGESAGAASVGMHILSLPSRSLFHRAVLQSGTPNGPWATVSAGEARRRATLLARLVGCPPGGAGGNDTELIACLRTRPAQDLVDHEWHVLPQESIFRFSFVPVVDGDFLSDTPEALINTGDFQDLQVLVGVVKDEGSYFLVYGVPGFSKDNESLISRAQFLAGVRIGVPQASDLAAEAVVLHYTDWLHPEDPTHLRDAMSAVVGDHNVVCPVAQLAGRLAAQGARVYAYIFEHRASTLTWPLWMGVPHGYEIEFIFGLPLDPSLNYTTEERIFAQRLMKYWTNFARTGDPNDPRDSKSPQWPPYTTAAQQYVSLNLKPLEVRRGLRAQTCAFWNRFLPKLLSATDTLDEAERQWKAEFHRWSSYMVHWKNQFDHYSKQERCSDL

Approximately 80 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.