Fomivirsen sodium  (Synonyms: ISIS-2922)

Fomivirsen (ISIS-2922) sodium is an antisense 21 mer phosphorothioate oligonucleotide. Fomivirsen sodium is an antiviral agent that is used cytomegalovirus retinitis (CMV) research, incluiding in AIDs. Fomivirsen sodium binds to and degrades the mRNAs encoding CMV immediate-early 2 protein, thus inhibiting virus proliferation^[1][2].

反义寡核苷酸与人巨细胞病毒主要早期区域蛋白的信使 RNA 互补，通过反义机制破坏病毒的复制^[1]。
Fomivirsen sodium 是21-mer 硫代磷酸酯 ASO，其 5' 末端附近带有 CpG 基序，序列为 5'-GCG TTT GCT CTT CTT CTT GCG-3'，通过 RNase H 介导的机制导致 mRNA 降解^[2]。
Fomivirsen sodium 以剂量依赖性方式抑制人视网膜色素上皮 (RPE) 细胞和人成纤维细胞 (MRC-5 细胞) 中的 CMV 复制。RPE 细胞的平均 IC₅₀ 为 0.03 μM，MRC5 细胞的平均 IC₅₀ 为 0.2 μM^[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

6682.4 (1mg=150nmol;5mg=748nmol)

160369-77-7

固体

White to off-white

DNA, d(P-thio)(G-C-G-T-T-T-G-C-T-C-T-T-C-T-T-C-T-T-G-C-G), eicosasodium salt

Room temperature in continental US; may vary elsewhere.

-20°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

• [1]. M D de Smet, et al. Fomivirsen- a phosphorothioate oligonucleotide for the treatment of CMV retinitis. Ocul Immunol Inflamm. 1999 Dec;7(3-4):189-98.  [Content Brief]

  [2]. Anna Kilanowska, et al. In vivo and in vitro studies of antisense oligonucleotides – a review. (Review Article) RSC Adv., 2020, 10, 34501-34516  [Content Brief]