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  3. Heparinase II

Heparinase II 是一种生化试剂,可作为生物材料或有机化合物,用于生命科学相关研究。

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Heparinase II Chemical Structure

Heparinase II Chemical Structure

CAS No. : 149371-12-0

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Customer Review

  • 生物活性

  • 纯度 & 产品资料

生物活性

Heparinase II is a biochemical reagent that can be used as a biological material or organic compound for life science related research.

体外研究
(In Vitro)

产品信息
钙离子会抑制肝素酶 II 活性。肝素酶 II 通过消除机制切割硫酸化多糖链,该多糖链含有 1-4 个连接,连接处为己糖胺和糖醛酸残基(包括艾杜糖醛酸和葡萄糖醛酸残基)。该反应生成含有不饱和糖醛酸的寡糖产物(主要是二糖),可通过 232 nm 的紫外光谱检测。该酶还可切割肝素分子中的抗凝血酶 III 结合五糖结构域。

溶解
可使用 1 mg/mL 溶于水,建议新鲜配制。或根据具体实验参考文献溶解。

反应条件
1. pH 稳定范围 5.0-9.0 ,最适 pH 7.0-7.5
2. 温度稳定范围 20-37℃,最适温度 30℃
注:酶切底物时需要根据实际经验确定孵育时间及酶浓度。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

CAS 号
性状

固体

颜色

White to off-white

中文名称

肝素酶Ⅱ

比活

≥2000 Units/mg protein

单位定义

One unit is defined as the amount of enzyme that will forms 0.1 μM unsaturated alduronic acid per hour at pH 7.5, 30°C.

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

纯度 & 产品资料

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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产品名称:
Heparinase II
目录号:
HY-P2955
需求量: